Member since: 2021
Organization: Peking university
Tecan's Spark Cyto is very good.
Application Area:Cellular fluorescence imaging
"Tecan's Spark Cyto is very good. It can automatically perform bright-field and fluorescence imaging of the cells in the well plate, and statistically output the results as needed. This greatly saves our time. At the same time, its fluorescence imaging signal is very strong and clear. It is much clearer than the general inverted fluorescence microscope. In addition, it can set temperature and carbon dioxide, and perform real-time detection of cells, making real-time tracking possible."
Member since: 2020
Organization: CMC HOSPITAL
Great one, I like it!
Application Area:Clinical lab
"This product is the first one that I liked and it has good value for money. It is also easy to use."
Member since: 2019
Organization: University of North Dakota
Great user friendly instrument, would highly recommend it!
Application Area:Drug development
"We have found that being able to do so many different applications within one product is great for our lab. We can do cell culture wound healing for drug development, nucleic acid analysis, cell counting, luminescence assays, colorimetric assays, etc. It has been extremely user friendly and has increased the efficiency and reliability of our experiments."
Member since: 2017
Organization: Nerviano Medical Sciences
The Spark Cyto can be an excellent support in any cell biology laboratory.
Application Area:Phenotypic screening, cell-based assay development, target identification...
"Using the Spark Cyto for long term proliferation experiments I was impressed by its capabilities for automated cell incubation and analysis: we observed that the cells grow very homogeneously and with a similar doubling time compared to a dedicated cell incubation approach. The Spark Cyto is equipped with excellent optics, an ultra-sensitive camera and I particularly appreciate the presence of a 2x objective which allows perfect whole-well imaging at cellular resolution in a 96-well plate. The imaging capability of this instrument, both in bright field and in multiple fluorescence channels, allows reproducible analysis in real time from live cell cultures. The flexibility and ease of use of this instrument make it particularly suitable for both cell-based assay development and for medium-high throughput phenotypic screening. The Spark Cyto represents, in my opinion, an excellent support for daily activities in any cell biology laboratory."
Member since: 2017
Organization: Univ. of Graz
Can be recommended to labs with many students.
Application Area:Cell survival assays, ELISA
"Using the Spark Cyto for cell imaging, enzymatic tests and immunoassays, we were impressed by how intuitive and easy to use the software is. Our diploma and PhD students instantly learned how to work with Spark Cyto and obtain high quality results."
Member since: 2019
Organization: Leibniz-Forschungsinstitut für Molekulare Pharmakologie
Great system with broad detection portfolio. Ideal for assay development and screening!
Application Area:HTS for drugs (70.000 compounds) and gene function (RNAi & CRISPR-Cas)
"This detection system nicely bridges the gap between a plate reader and imaging systems like automated microscopes. It combines multi parameter screening (all kinds of fluorescence detection) with the features of image based screening (cytometric measurements) in one machine. Coupling kinetic measurements over long time periods to injection of reagents at the most effective time points within the reader. Ideal system to be used in assay optimisation and in screening. Nice graphical interface for assay set up and a lot of nice analysis features like confluence or viability measurements."
Spark Cyto builds on the foundation of the original Spark platform, combining the flexibility of a high-end multimode plate reader with whole well imaging and comprehensive environmental control to allow multiparametric measurements for cell-based applications.
This dynamic instrument uses real time data acquisition and analysis to deliver meaningful insights faster than ever before, ensuring that no key event is ever missed. It combines live cell imaging with industry-leading detection technologies, allowing qualitative and quantitative information to be integrated into unique multiparameter data sets. Top-of-the-range camera components and patent-pending LED autofocus technology offer three magnification levels and four acquisition channels (for fluorescence and bright field imaging), allowing the investigation of entire cell populations, and enabling the whole well area of a 96- or 384-well microplate to be recorded with just one image, without tiling or distortion.
Spark Cyto is designed to handle a broad range of common cytometry applications, offering a new level of experimental control without compromising on ease of use and convenience. Powerful SparkControl™ and Image Analyzer™ software give the operator complete control of all the parameters associated with their experiment, with five predefined methods to offer straightforward image acquisition and analysis for common cytometry applications. These are complemented by additional features – such as ‘user-defined’ parameters and Real Time Experimental Control (REC™) to enable kinetic experiments to be performed automatically – to deliver multiplexed live cell data from a single instrument, unlocking new possibilities for cell-based research and giving increased confidence in the statistical relevance of the results.
Spark Cyto is available in four specialized configurations with different detection modalities, each fully equipped for real-time live cell imaging cytometry.
Please note that Spark Cyto is for research use only.