Automated 3D cell culture and drug testing using Corning Matrigel Matrix
1 Jan 2025This application note details an automated workflow for 3D cell culture. It includes controlled pipetting of Matrigel® matrix, cell seeding and spheroid cultivation using a Fluent Automation Workstation with an integrated, liquid-cooled four-position carrier and a Thermo Scientific™ Cytomat™ 2 C450-LiN automated incubator. Drug treatments were performed using the D300e Digital Dispenser. A Spark® Cyto multimode reader was used to monitor cell growth by brightfield imaging, as well as to perform luminescence-based viability assessment using the CellTiter-Glo® 3D Cell Viability Assay (Promega).
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