Automated 3D cell culture and drug testing using Corning Matrigel® Matrix

This application note details an automated workflow for 3D cell culture. It includes controlled pipetting of Matrigel matrix, cell seeding and spheroid cultivation using a Fluent Automation Workstation with an integrated, liquid-cooled four-position carrier and a Thermo Scientific™ Cytomat™ 2 C450-LiN automated incubator. Drug treatments were performed using the D300e Digital Dispenser. A Spark Cyto multimode reader was used to monitor cell growth by brightfield imaging, as well as to perform luminescence-based viability assessment using the CellTiter-Glo® 3D Cell Viability Assay (Promega).