ResourceSeparations
Prep SFC as a complementary tool to prep HPLC
4 Nov 2024To achieve the widest application range of chromatography, harnessing the power of two separation techniques, supercritical fluid chromatography (SFC) and high-performance liquid chromatography (HPLC), is preferable. HPLC is known as an all-rounder for most applications, whereas SFC offers increased speed, environmental benefits, and superior performance for chiral separations. Explore how these complementary approaches enhance the versatility and efficiency of chromatography, enabling more comprehensive and robust analysis in various research and industrial applications.
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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Supercritical Fluid ChromatographySupercritical Fluid Chromatography (SFC) is used for the rapid separation and analysis of complex mixtures. SFC systems require injectors, pumps, columns, ovens and detectors. The SFC retention method uses a supercritical fluid, usually CO2. There are a number of different SFC detector types including UV / VIs, ECD, mass spectrometry and fluorescence.Liquid Chromatography