Label-free quantification of tumor spheroid drug responses in InSphero ARCTis™ Plates

This Tecan application note demonstrates how label-free imaging can be used to monitor tumor spheroid drug responses in InSphero ARCTis™ Plates. Using the Spark® Cyto imaging microplate reader, HCT 116, T-47D, and A549 tumor spheroids were treated with varying concentrations of Staurosporine and monitored over time to assess dose-dependent changes in spheroid area.

Learn how automated brightfield imaging and 3D AI-based analysis can support kinetic monitoring of spheroid growth and treatment response without the need for labeling. The workflow is compared with endpoint CellTiter-Glo® 3D viability measurements and live/dead staining, showing how label-free imaging can provide complementary insight into spheroid viability, morphology, and drug response dynamics.

Discover how Spark Cyto can help streamline 3D cell culture analysis by enabling automated, label-free monitoring of physiologically relevant tumor models for high-throughput screening and translational research.

Resource details:
Resource type: Application note
Page count: 5
Read time: 8 mins