Freedom EVO® for Protein Purification
Tecan has partnered with GE Healthcare to develop an automated solution for screening of best producing clones, using MultiTrap plates on the popular Freedom EVO platform.
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MultiTrapTM plates are 96-well format plates, prepacked with chromatography media for His-/GST-tagged proteins, and are fully tested for screening of recombinant proteins on the Freedom EVO.
The combination of Tecan's robotic integration and liquid handling expertise and the versatile MultiTrap platform provides users with a fully automated protein purification system for higher throughput demands. Proteins can be extracted from E. coli cultures without centrifugation or filtration, creating a more efficient workflow. Purification can be carried out either under vacuum pressure or by centrifugation.
The system allows processing of both His- and GST-tagged fusion proteins, with low cross-contamination, high reproducibility and highly robust processing. MultiTrap plates are also available for screening of antibodies, parallel enrichment of the target protein – such as protein A and streptavidin – by immuno-affinity and desalting/buffer exchange. Purification can be easily scaled up on the ÄKTAxpress™ system from GE Healthcare.
Fast and Efficient PCR Clean-Up with the NucleoSpin<sup>®</sup> 96 PCR Clean-Up Kit on a Freedom EVO<sup>®</sup> Platform
This application note demonstrates how MACHEREY-NAGEL’s NucleoSpin® 96 PCR Clean-up kit and Tecan’s Freedom EVO® platform can be used in combination to provide a flexible automated solution for the purification of PCR products. PCR has become a standard molecular biology tool for biological and medical research laboratories, and is used for DNA cloning, sequencing and genetic testing. For most applications, PCR products need to be purified, and this is advantageous to perform in a high throughput format.
