Real-time growth monitoring of spheroids in round bottom microplates

Three-dimensional (3D) cell culture systems have emerged as promising techniques in the field of cell-based assays, with approaches such as multicellular 3D spheroids (dense cell aggregates mostly comprised of cell lines) gaining widespread attention. Different methods of spheroid generation have been explored, such as magnetic levitation, hanging drop methods, and the use of ultra-low attachment (ULA) round bottom microplates for scaffold-free generation of 3D aggregates independent of solid carriers. Tecan describes the generation of spheroids in Corning 96-well, black, round bottom, ultra-low attachment microplates, and monitoring of spheroid growth in the Spark^® Cyto multimode reader using brightfield (BF) and fluorescence (FL) imaging. Discover how the Spark Cyto provides ease of use, multiplexing capabilities, and the possibility of executing an entire workflow on one instrument.

Please note that Spark Cyto is for research use only. Not for use in diagnostic procedures.