p217 Tau Luminescence Immunoassay
The p217 Tau LUM is a sensitive research tool that together with an upfront BD-Tau immunoprecipitation allows for the measurement of p217 BD-Tau in blood samples.
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The Tau protein is a microtubule-stabilizing protein being part of the axons of nerve cells in the central nervous system (CNS). Measuring total Tau in CSF reflects axonal destruction and has found its way into diagnostic criteria guidelines¹, but measuring total Tau in blood does not correlate with its corresponding CSF value. As 80% of Tau in blood originates from peripheral tissue, being able to selectively measure the brain-derived fraction will enhance specificity and accessibility of Tau as an AD biomarker.
p217 Tau has been shown to correlate with the Amyloid-beta pathology in Alzheimer's disease patients and can discriminate between so-called amyloid-beta-positive and amyloid-beta-negative patients (determined by CSF amyloid-beta measurement)
Automated BD-Tau and p217 Tau analysis on the Fluent 780 Workstation
This Tecan application note presents a fully automated workflow for enriching and quantifying brain-derived Tau (BD-Tau) and phosphorylated p217 Tau from blood samples using the Fluent® 780 Workstation. By integrating immunoprecipitation with luminescence-based measurement, the workflow supports reliable analysis of blood-based biomarkers relevant to Alzheimer’s disease and neurodegenerative disease research.
Discover how the automated protocol reduced hands-on time from more than four hours to just 20 minutes per 96-well plate. It also improved precision compared with manual processing, showed no significant plate drift, and enabled BD-Tau and p217 Tau measurement from the same enriched eluate.
Resource details
Resource type: Application note
Page count: 9
Read time: 14 mins
