ACQUITY Premier System

Environmental contamination by per- and polyfluoroalkyl substances (PFAS) and rising concerns about its long-term impacts on the general population, have increased the urgency for wider PFAS analysis. Initially the focus was solely on environmental matrices but has now expanded into food analysis due to the risk to consumers. Accordingly, regulations and guidance documents have been released to assure the quality of water bodies, drinking water and most recently also for food and feed. Here, Waters describes the workflow of its sample-to-result approach for PFAS analysis that can be incorporated into any lab whether the focus is food, water or environmental.

Download the latest eBook published by Waters for three fresh perspectives on the capabilities and adoption trends of MAM workflows within the biopharmaceutical industry.

In this application note, Waters highlights a method for the separation of fourteen organic acids using the ACQUITY UPLC H-Class PLUS and ACQUITY QDa Mass Detector.

In this application note, successful method adaptation for multiple amino acid application areas, including those amino acids found in protein hydrolysate, cell culture, food and feed, and alkylated cysteines samples, from the ACQUITY UPLC to the ACQUITY Premier Binary System was demonstrated. The critical performance characteristics were maintained after method adaptation.

In this application note, Waters demonstrates how the ACQUITY PREMIER System with hybrid organic surface technology was able to reduce the binding of many analyte molecules, such as phosphates, uncharged amines, hydroxyls, and deprotonated carboxylic acids, with the transition metal ions present on the metal surfaces in the chromatographic system.

In this application note, Waters demonstrates two different test approaches to successfully demonstrate the attenuation of metal-binding in the inert ACQUITY PREMIER System when compared to the standard ACQUITY UPLC H-Class PLUS Bio System. Increases in absolute peak area and improvements in peak shape were also observed. With respect to the AMPcP and adenosine standard and testing via a chromatographic separation, it was also found that the ACQUITY PREMIER System provided significantly higher peak area ratios for AMPcP/adenosine and thus higher recoveries of metal-sensitive analytes.

In this application note, Waters investigates the benefits of MaxPeak HPS technology for the analysis of phosphopeptides. Waters demonstrates how the MaxPeak HPS hardware takes advantage of a hybrid inorganic/organic silica surface layer which is chemically similar to bridged-ethyl hybrid silica. When applied to metallic surfaces wetted during an LC separation, such as the column and LC fluidic path, MaxPeak HPS imparts a resilient barrier but does not participate in separation of sample constituents. Experiments were performed in which the recovery response of numerous phosphopeptides were measured using both conventional and HPS-modified systems.

In this application note, Waters demonstrates the benefits of MaxPeak HPS Technology using adenosine and its phosphorylated derivatives as test analytes and shows a case study on when and how chromatographic surfaces can be of significant influence to analyte detection.

In this application note, Waters shows the impact of using a novel surface for quantifying hydrocortisone phosphate and dexamethasone phosphate in extracts of human plasma. Using the ACQUITY PREMIER System and MaxPeak HPS Columns, Waters were able to achieve a 10-fold improvement in the lowest limit of quantification (LLOQ) for hydrocortisone phosphate and 7.5-fold increase in LLOQ for dexamethasone phosphate.

In this application note, Waters demonstrates that the analysis of the TCA cycle and related metabolites in human plasma can be achieved with great analytical sensitivity when incorporating MaxPeak HPS technology into the liquid chromatograph as well as the analytical column. The ACQUITY PREMIER System mitigates analyte interactions with metal to improve peak shape as well as analytical sensitivity without full-system passivation with strong acids or chelating additives in the mobile phase.

In this video, discover how the ACQUITY Premier Solution with MaxPeak High Performance Surfaces Technology is designed to reduce adsorption of oligonucleotides with metal surfaces, improving quantification, sensitivity, accuracy, and robustness compared to a standard UPLC system and column.

In this video, hear testimonials from employees at the Waters Corporation Milford Machine Shop on what quality means for them and discover why the quality of the products they are producing is vital to their drug discovery customers' success.

In this video, Waters discusses how the ACQUITY PREMIER system helps your lab overcome limited sensitivity, poor reproducibility, and missing analytes as well as increasing your lab's confidence in your chromatography workflow.

Watch this on-demand webinar series to discover innovative liquid chromatography solutions designed to provide ultimate performance, particularly for metal-sensitive analytes

Watch this free on-demand webinar to uncover the benefits of a bioinert LC system for biopharmaceutical-based separations

The first-of-its-kind software for TA’s thermal analysis product line helps customers save time and money by automating standard operating procedures

Explore top tips for oligonucleotide analysis and learn how to optimize your sample prep and LC-MS acquisition

Watch this on-demand webinar to learn about the advantages of a new hybrid surface technology in mitigating problematic metal interactions

Discover how a universal solution for non-specific adsorption could improve the sensitivity and reproducibility of your analyses while saving time and effort