ResourceDrug Discovery & Development
Achieving effective biotherapeutic peptide and protein size-based analyses while maximizing SEC column life
20 Aug 2026Success with size exclusion chromatography (SEC) depends on more than choosing the right column. System dispersion, unwanted interactions, contamination, and poor handling practices can all undermine peptide and protein analyses, affecting both data quality and column lifetime.
In this resource, discover how you can optimize SEC workflows for biotherapeutic peptides and proteins. Learn how to improve separations, extend column life, and avoid common analytical pitfalls, while gaining practical guidance to support more reliable results and greater confidence in your biotherapeutic characterization workflows.
Resource details:
Resource type: eBook
Page count: 31
Read time: 48 mins
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Size Exclusion ChromatographySize exclusion chromatography (SEC) is a liquid chromatographic technique used to separate and identify molecules based on their size, including polymers, proteins, nanoparticles & nucleic acid. SEC is further subdivided into gel filtration chromatography (GFC) and gel permeation chromatography (GPC) when an aqueous or organic mobile phase is used respectively. HPLC-based SEC systems and columns are available for analytical separation alongside SEC spin columns or filters to prep samples for many protocols. Find the best size exclusion chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.BiopharmaceuticalsBiopharmaceuticals are proteins and other compounds (such as nucleic acids) produced by living organisms that have uses as therapeutics or for in vivo diagnostics. The most well known example of a biopharmaceutical product, and the first to be approved for therapeutic use, was recombinant human insulin.
