Gene Expression and Molecular Cloning Products & Reviews

Prepare Cap 1-RNA from any source of Cap 0-RNA. Use simultaneously with ScriptCap™ m7G Capping System to produce virtually 100% Cap 1-capped RNA in a single reaction.

Generate pseudouridine-containing mRNA (Ψ-mRNA) with greatly reduced double-stranded RNA (dsRNA) content. Easily remove dsRNA without the need for chromatographic methods like HPLC. Produce large amounts of Ψ-mRNA with virtually 100% transcript capping. Induce lower innate immune response in mammalian cells.

Specially formulated for maximum yields of canonical (GAUC) in vitro transcribed RNA. Produce up to 180 µg of RNA from 1 µg of template in just 30 minutes. Easily scale up reactions to produce milligram amounts of RNA.

Optimized for high-yield synthesis of 5-methyl-cytidine- pseudouridine-containing, in vitro transcribed RNA (5mCΨ-RNA). Produce up to 100 µg of 5mCΨ-RNA from 1 µg of template in 2 hours. Easily scale up to produce milligram amounts of 5mCΨ-RNA. Induce lower innate immune responses in mammalian cells by using 5mCΨ-mRNAs.

Quantitative mRNA poly(A) tail length using standard polyacrylamide gel electrophoresis (PAGE). Easy, cost-effective benchtop method for QC as an alternative to mass spec analysis. Assess tail lengths above the limits of HPLC and LC-MS based methods.

Quantitate mRNA capping efficiency (percent capped RNA content) of XBG 5’ UTR-containing mRNA using standard polyacrylamide gel electrophoresis (PAGE). XBG 5’ UTR Targeting Oligonucleotide and XBG 5’ UTR Control Mix included in kit. Easy, cost-effective, benchtop method useful for QC as an alternative to HPLC and LC-MS analysis.

Easy to use, scalable enzymatic method for removing double-stranded RNA (dsRNA) from RNA samples. Remove dsRNA without the need for chromatographic methods like HPLC. No reduction in single-stranded RNA (ssRNA) yields. Reduces the innate immunogenic response to mRNA in cells.

Inhibits eukaryotic RNases A, B and C. Use as a safeguard against RNA degradation in any RNA-containing reaction.

Optimized for high-yield synthesis of pseudouridine-containing, in vitro transcribed RNA (Ψ-RNA). Produce up to 100 µg of Ψ-RNA from 1 µg of template in 2 hours. Induce lower innate immune responses in mammalian cells by using Ψ-mRNA.

All-in-one kit to produce N1-methyl-pseudouridine-containing-mRNA (N1meΨ-mRNA). Combine the benefits of high-yield transcription with post-transcriptional capping and tailing. Produce large amounts of N1meΨ-mRNA with virtually 100% transcript capping. Induce lower innate immune response in mammalian cells by using N1meΨ-mRNAs.

Specially formulated for maximum yields of pseudouridine-containing, in vitro transcribed RNA (Ψ-RNA). Produce up to 160 µg of Ψ-RNA from 1 µg of template in 1 hour. Easily scale up to produce milligram amounts of Ψ-RNA. Induce lower innate immune responses in mammalian cells by using Ψ-mRNAs.

Optimized for high-yield synthesis of pseudouridine-containing, in vitro transcribed RNA (Ψ-RNA). Produce up to 60 µg of Ψ-RNA from 1 µg of template in 2 hours. Easily scale up to produce milligram amounts of Ψ-RNA. Induce lower innate immune responses in mammalian cells by using Ψ-mRNA.

For post-transcriptional poly(A) tail addition to RNAs. Tail mRNAs in vitro to lengths equivalent to or greater than those found in nature.

Co-transcriptionally cap 5-methyl-cytidine- pseudouridine-containing, in vitro transcribed RNA with Anti-Reverse cap analog (ARCA). Produce up to 40 µg of capped 5mCΨ-RNA from 1 µg of template in 3 hours. Optimized for maximum transcript capping and yield. Induce lower innate immune responses in mammalian cells by using 5mCΨ-mRNAs.

Quantitate mRNA capping efficiency (percent capped RNA content) using standard polyacrylamide gel electrophoresis (PAGE). Easy, cost-effective, benchtop method useful for QC as an alternative to HPLC and LC-MS analysis.

Quantitate the percentage of mRNA with Cap 1 vs Cap 0 5’-cap structures using standard polyacrylamide gel electrophoresis (PAGE). Easy, cost-effective benchtop method for QC as an alternative to mass spec analysis.

All-in-one kit to produce pseudouridine-containing-mRNA (Ψ-mRNA). Combine the benefits of high-yield transcription with post-transcriptional capping and tailing. Produce large amounts of Ψ-mRNA with virtually 100% transcript capping. Induce lower innate immune response in mammalian cells by using Ψ-mRNAs.

Specially formulated for maximum yields of N1-methyl-pseudouridine-containing, in vitro transcribed RNA (N1meΨ-RNA). Produce up to 160 µg of N1meΨ-RNA from 1 µg of template in 1 hour. Easily scale up reactions to produce milligram amounts of N1meΨ-RNA. Induce lower innate immune responses in mammalian cells by using N1meΨ-mRNAs.

Completely hands-off, walk-away HCP analysis in 1 hour.

The SpectraMax® DuoLuc™ Reporter Assay Kit enables highly sensitive quantitation of both firefly and Renilla luciferases in mammalian cells at a great price. Flash-type luminescence with excellent sensitivity Expression measurement in as few as ten cells per well Improved accuracy of results by normalizing reporter expression to a control Optimized for SpectraMax® i3x, iD3, or L microplate readers with injectors…

Gene Pulser®/Micropulser™ electroporation cuvettes are high-quality electroporation cuvettes which enable reproducible and consistent gene delivery to valuable transfection samples.

Pkg of 100, bursts at 1,800 psi in a partial vacuum, for use with the PDS-1000/He biolistic system (#165-2257, #165-2258)

Pkg of 100, bursts at 1,550 psi in a partial vacuum, for use with the PDS-1000/He biolistic system (#165-2257, #165-2258)

Pkg of 100, bursts at 1,100 psi in a partial vacuum, for use with the PDS-1000/He biolistic system (#165-2257, #165-2258)

Pkg of 100, bursts at 900 psi in a partial vacuum, for use with the PDS-1000/He biolistic system (#165-2257, #165-2258)