Gene Expression and Molecular Cloning Products & Reviews

Guaranteed editing with predesigned gRNA designs

Use our proprietary algorithms to design custom Cas9 guide RNAs

Guide RNAs (gRNAs) contain the target-specific sequence for guiding Cas9 protein to a genomic location.

Guide RNAs (gRNAs) contain the target-specific sequence for guiding Cas9 protein to a genomic location.

Guide RNAs (gRNAs) contain the target-specific sequence for guiding Cas9 protein to a genomic location.

Hybridizes to crRNA to activate the Cas9 enzyme.

Hybridizes to crRNA to activate the Cas9 enzyme.

urified carrier DNA that improves delivery of Cas9 ribonucleoprotein (RNP) by electroporation.

Purified carrier DNA that is required for efficient delivery of Cpf1 ribonucleoprotein (RNP) by electroporation.

For detection of on-target or known off-target CRISPR events in cultured cells

An advanced data analysis pipeline for quantifying your genome editing results

A small molecule compound that has demonstrated an ability to increase the rate of homology-directed repair (HDR).

Deep, targeted amplicon sequencing with highly multiplexed panels for sequencing on Illumina platforms.

Complete synthetic genes with 100% sequence verification are provided in a plasmid cloning vector and ready to use in a variety of applications.

Double-stranded DNA fragments for quick and cost-effective cloning

Hybridizes to crRNA to activate the Cas9 enzyme.

Hybridizes to crRNA to activate the Cas9 enzyme.

NanoLuc® and NanoBiT® Luciferase Substrate for Animal Imaging Studies​

Human iPSC-derived microglia expressing Cas9 for rapid gene knockout generation

Fast-track your route to a modernized workflow with the only in vitro bioavailability assessment tool on the market

FOPflash: Transfection grade T cell factor (TCF) reporter plasmid containing two full and one incomplete copy of the TCF binding site (mutated) followed by three copies in the reverse orientation, upstream of the Thymidine Kinase (TK) minimal promoter and Luciferase open reading frame. This plasmid serves as a negative control to TOPflash which contains wild type TCF binding sites.

TOPglow (TCF reporter plasmid): Transfection grade T cell factor (TCF) reporter plasmid containing four copies of the Tcf binding site (wt) upstream of E1B TATA box and Luciferase open reading frame.

The Mammalian Cell Transfection Kit is an optimized system suitable for the introduction of DNA into mammalian cells. DNA is introduced to the cells as a calcium phosphate precipitate. Transfected cells integrate and express the exogenous DNA at a higher efficiency, producing stable clones of the specifically altered genotype and phenotype. This system has been used to introduce various plasmids into a broad range of cell line…

A highly efficient method of gene trasfer into mammalian cells is through infection with retroviral vectors. The efficiency of retroviral infection is enhanced significantly, 100 to 1,000 fold in some cells, by including polybrene during the infection. Polybrene with DMSO shock is also used to mediate DNA transfer into a variety of cell types, such as: CHO, chicken embryo fibroblases, NIH3T3 cells, and myeloid cells.