ScriptCap™ m⁷G Capping System
Build Cap 0 caps with nearly 100% efficiency. Use simultaneously with ScriptCap™ 2'-O-Methyltransferase to produce Cap 1 caps.
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The ScriptCap™ m7G Capping System catalyzes in vitro addition of a cap nucleotide to the 5' terminus of primary RNA that has a 5'-triphosphate group, such as RNA obtained from an in vitro transcription reaction, at approximately 100% efficiency. A "cap nucleotide" or “cap” is a guanine nucleoside that is joined via its 5' carbon to a triphosphate group that is, in turn, joined to the 5' carbon of the most 5' nucleotide of the primary mRNA transcript, and in most eukaryotes, the nitrogen at the 7 position of guanine in the cap nucleotide is methylated. This system can also be used simultaneously with the CELLSCRIPT™ ScriptCap™ 2'-O-Methyltransferase Kit to produce Cap 1 caps. Product is for research use only (RUO).
