INCOGNITO™ T7 mScript™ Complete N1meѰ-mRNA Production System
Generate N1-methyl-pseudouridine-containing mRNA (N1meΨ-mRNA) with greatly reduced double-stranded RNA (dsRNA) content. Easily remove dsRNA without the need for chromatographic methods like HPLC. Produce large amounts of N1meΨ-mRNA with virtually 100% transcript capping. Induce lower innate immune response in mammalian cells.
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The INCOGNITO™ T7 mScript™ Complete N1meѰ-mRNA Production System provides all enzymes and enzyme-related reagents for making N1-methyl-pseudouridine-containing, 5'-capped, 3'-polyadenylated mRNA with greatly reduced double-stranded RNA content, lower than what can be achieved with special T7 RNA polymerase mutants. The kit includes modules for in vitro transcription of linear double-stranded DNA templates using the T7 mScript™ Enzyme Solution, the canonical nucleotides ATP, CTP, GTP and the modified nucleotide N1-methyl-pseudouridine-5'-triphosphate, enzymatic capping of the RNA using the ScriptCap™ Cap 1 Capping System that contains both ScriptCap™ Capping Enzyme and 2'-O-Methyltransferase for making mRNA with a Cap 1 cap structure, A-Plus™ Poly(A) Polymerase for adding a 3'-poly(A) tail, enzymatic removal of dsRNA content using the Min-Immune™ Gold dsRNA Removal system and 5 M NH4OAc as a convenient RNA/mRNA purification method. Kit includes a limited non-exclusive, non-transferable label license to use the products purchased from CELLSCRIPT™ solely for purchaser's own internal laboratory research use. Product is for research use only (RUO).
