Antisense Oligonucleotides
Modulation of gene expression with modified oligos
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Antisense oligonucleotides (ASOs) are short, single-stranded nucleic acid molecules that can selectively bind to target RNA molecules, resulting in the regulation of gene expression. ASOs are a promising approach for gene expression and silencing studies. They can be designed to inhibit the expression of disease-causing genes or enhance the expression of beneficial genes.
ASOs work by binding to the complementary RNA sequence, forming a hybrid double-stranded molecule. This interaction can result in various outcomes, including modulation of RNA stability, inhibition of RNA translation, or recruitment of cellular machinery for targeted degradation of the RNA molecule. ASOs are used in research laboratories for studying gene function, understanding disease mechanisms, and developing new therapies.
Read all about ASOs in this technical article, including modulation mechanisms, designs considerations, different generations of constructs, and quality considerations. Seek design assistance and order ASOs with a variety of modifications that facilitate stability.
Antisense oligonucleotides: Modulation of gene expression with modified oligos
Traditional antisense oligonucleotides (ASO)-based gene modulation (usually synonymous with silencing or downregulation of gene expression, but it can be used to improve gene expression and, in at least one particular case, it was shown to lead to upregulation of gene expression) targets mRNA and can take place in either the nucleus or the cytoplasm. Gene silencing should be simple, but there are many considerations to take into account for a successful ASO design. In this application note, Merck presents ASOs for modulation of gene expression. The note highlights common types of ASO modification and provides considerations for different types of experiments.
