MDCK Cell Line

Application

• Virus studies: SVEV, VSV, Vaccinia, reoviruses and inf. canine hepatitis virus

Biochem/physiol Actions

• Canine Cocker Spaniel kidney
• Derived from the kidney of a normal adult Cocker Spaniel in 1958. Useful in viral studies, supports the growth of vesicular exanthema virus of swine, vesicular stomatitis, vaccina, coxsackie B-5, adenoviruses, reoviruses and infectious canine hepatitis. Also known as MDCK (NBL-2).

Preparation Note

• EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10%
• Foetal Bovine Serum (FBS).
• Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Cells attach firmly and require at least 2 PBS washes prior to addition of trypsin/EDTA.