T7 mScript™ Complete Standard mRNA Production System
Generate canonical (GAUC) mRNA with greatly reduced double-stranded RNA (dsRNA) content. Easily remove dsRNA without the need for chromatographic methods like HPLC. Produce large amounts of mRNA with virtually 100% transcript capping. Reduce the innate immunogenic response to mRNA in cells.
The supplier does not provide quotations for this product through SelectScience. You can search for similar products in our Product Directory.
The T7 mScript™ Complete Standard mRNA Production System provides all enzymes and enzyme-related reagents for making canonical uridine-containing, 5'-capped, 3'-polyadenylated mRNA with greatly reduced double-stranded RNA content, lower than what can be achieved with special T7 RNA polymerase mutants. The kit includes modules for in vitro transcription of linear double-stranded DNA templates using the T7 mScript™ Enzyme Solution and the canonical nucleotides ATP, CTP, GTP and UTP, enzymatic capping of the RNA using the ScriptCap™ Cap 1 Capping System that contains both ScriptCap™ Capping Enzyme and 2'-O-Methyltransferase for making mRNA with a Cap 1 cap structure, A-Plus™ Poly(A) Polymerase for adding a 3'-poly(A) tail, enzymatic removal of dsRNA content using the Min-Immune™ Gold dsRNA Removal system and 5 M NH4OAc as a convenient RNA/mRNA purification method. Kit includes a limited non-exclusive, non-transferable label license to use the products purchased from CELLSCRIPT™ solely for purchaser's own internal laboratory research use. Product is for research use only (RUO).
