CRISPR/Cas9 Off-target Validation Solution
CRISPR off-target effect may result in anomalous insertions, deletions and translocation. It is critical to have a sensitive detection tool for the off-target editing. Based on the highly sensitive, accurate and stable TargetSeq® hybridization capture sequencing technology and MultipSeq® multiplex amplicon sequencing technology, iGeneTech provides a new, fast and cost-effective solution for CRISPR off-target validation to dete…
Receive your quote directly from the manufacturer.
Advantages
- Professional and fast panel customized solution
- Proven target sequencing technologies with excellent performance and simple workflow
- Mutation detection limit as low as 1%
- Identification of off-target sites is fast, accurate and cost intensive.
Cases
Factors determining editing efficiency and off-target cleavage activity for sgRNA
Researchers performed a study to understand the poorly documented factors that determine the editing efficiency and off-target cleavage activity of sgRNA by comparing the in-silico prediction and sequencing results from customized TargetSeq® panel. The result suggested that sgRNA activity and specificity can be sensitively detected by TargetSeq in combination with in-silico prediction.
