IGT® Enzyme Plus Library Prep Kit V3
IGT® Enzyme Plus Library Prep Kit V3 provides you a fast and streamlined workflow for NGS library preparation with enzymatic fragmentation strategy. The kit enables tunable fragmentation for different insert sizes according to experimental requirements and is suitable for genomic DNA and FFPE DNA samples with ranged sample input to generate satisfied yield. To achieve good performance with increased sequencing efficiency, IGT®…
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Advantages
- Suitable for library construction with a wide range of sample input (5~500ng)
- Compatible with varies of sample types
- Stable and efficient library yield with tolerance to EDTA
- Lower artifact rate with higher mutation detection accuracy
Performance
Consistent Performance with Different Sample Input
The IGT® Enzyme Plus Library Prep Kit V3 is compatible with different DNA inputs for library construction. Following the instruction for recommended PCR cycles, you can easily generate library with high yield and stable size.
Consistent Performance with Different Sample Input
Tunable Insert Size
The IGT® Enzyme Plus Library Prep Kit V3 is capable to generate desired library inset size by simply varying the enzymatic fragmentation time according to different requirements. The library yield is stable and sufficient for subsequent experiments.
Tunable Insert Size
Tolerance to EDTA
The IGT® Enzyme Plus Library Prep Kit V3 could tolerate EDTA with final concentration of 0.2 mM, which will have limited influence on enzymatic fragmentation. For higher EDTA concentration, we provide Enhancer Buffer E v3 for neutralization and you may use it according to the protocol instruction.
Tolerance to EDTA
Lower Artifact Rate
The IGT® Enzyme Plus Library Prep Kit V3 could have a lower artifact rate compared to other commercial available products, which significantly preserve the sequencing efficiency and accuracy.
Lower Artifact Rate
Accurate Detection of Mutations
According to the analysis of detected mutations of NA12878 reference standard samples, the results were highly consistent among repetitions of library construction using IGT® Enzyme Plus Library Prep Kit V3, indicating its high accuracy.
Excellent Performance for Target Enrichment
The IGT® Enzyme Plus Library Prep Kit V3 can be used with hybridization-based capture workflow. The tests with TargetSeq® panels of different panel sizes showed excellent performance in capture rate, coverage rate and uniformity.
