TargetSeq® Universal Blocking Oligo
TargetSeq® Universal Blocking Oligo series products are used to block self-ligation of adapters to reduce non-specific adapter interaction during probe hybridization and thus to increase data utilization. iGeneTech offers 2 versions of universal blocking oligo for different library input in hybridization workflow, i.e., TargetSeq® Universal Blocking Oligo and TargetSeq® Eco Universal Blocking Oligo for up to 6 μg and 3 μg libr…
Receive your quote directly from the manufacturer.
Advantages
- Suitable for various types of libraries
- Stable and excellent performance with different panels and inputs
- Pre-mixed reagents for simple operation.
Library Types
- Illumina
- MGI DI
- MGI SI
Performance
Excellent blocking effect for different library types
TargetSeq® Universal Blocking Oligo series products are suitable for different library types. The following figures are test results for single- and dual-indexed libraries with or without UMI, and the results showed that the performance are stable and excellent in all tested library types.
Excellent blocking effect for different library types
Stable performance for panels with different region sizes
TargetSeq® Universal Blocking Oligo series products work well with panels of different region sizes. The results are shown in the figures below for small, median and large panels of region size of 70 kb, 1 Mb and 34 Mb, respectively.
Stable performance for panels with different region sizes
Stable performance with different input amounts
TargetSeq® Universal Blocking Oligo series products are stable for different library input amounts. In a test with different input library amount of 750 ng, 3 μg and 6 μg, the results were stable among all samples.
Stable performance with different input amounts
Stable performance with multiplexing
TargetSeq® Universal Blocking Oligo series products performed stable and well in multiplexing testing with 1-Plex (500 ng), 6-Plex (3 μg) and 12-Plex (6 μg).
