MACSQuant® Tyto® Cell Sorter
The MACSQuant ® Tyto ® is a next generation, benchtop cell sorter equipped with 3 lasers, which allows for high speed, 10-parameter cell sorting. A unique feature of the instrument is the fact that the actual sorting process takes place exclusively within a single-use, disposable and fully closed system: the MACSQuant Tyto Cartridge. The closed cartridge provides full operator safety and eliminates the risk of sample contamin…
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If you need to sort rare cells for cell culture, this machine is what you need.
Sorting rare non-hematopoietic stem cells from human cord blood samples
Cord blood contains multiple stem cell populations, both hematopoietic and non-hematopoietic. We are interested in purifying lineage-negative CD34-negative cells which express CD31 as well a number of embryonic and induced pluripotent stem cell antigens. These cells are very rare, constituting ~ 0.2 – 0.3% of cord blood mononuclear cells. The cells are small with very little cytoplasm and their nuclei have a blast cell morphology. The sorting strategy is Hoechst 33342+ to gate on 2n cells, then gating on CD45 low vs lineage-negative and sorting CD31+ cells. We have sorted these cells both on the Tyto and a Becton-Dickinson Influx machine. We find the purity and yield of our sorts are comparable between the machines, with higher viability on the Tyto-sorted samples. Tyto-sorted samples are concentrated during the sorting, while Influx-sorted samples are not, and the unsorted population from Tyto runs can be resorted to increase the yield, which we have done one time, something that cannot be done on the Influx. The stability of the Tyto sort parameters is strikingly better than the Influx; the sorts are highly reproducible. Finally, the sealed sorting cartridge affords higher confidence of maintaining a sterile sort, which is crucial to our goal of developing a culture system for these cells. Other planned uses for the Tyto include sorting human hematopoietic stem cell subsets to characterize their activity, sorting peripheral blood lineages from allogeneic hematopoietic stem cell transplant patients to characterize lineage engraftment, and isolating genetically engineered lymphoid effector cell populations with the ultimate goal of use for patient cellular therapies.
Review Date: 22 Jan 2021 | Miltenyi Biotec B.V. & Co. KG
100% necessary for my experiment.
Cell sorting delicate CD8 mouse cells for in vivo transfer, 100% sterile
I used the Tyto multiple times to sterile sort mouse CD8 cells for an in-vivo experiment. The machine was easy to use and the remote support from Carter Melton at Miltenyi has been consistently superb. The output product has always been sterile and of high purity. Would 10/10 recommend.
Review Date: 19 Jan 2021 | Miltenyi Biotec B.V. & Co. KG
The MACSQuant® Tyto® is a next generation, benchtop cell sorter equipped with 3 lasers, which allows for high speed, 10-parameter cell sorting. A unique feature of the instrument is the fact that the actual sorting process takes place exclusively within a single-use, disposable and fully closed system: the MACSQuant Tyto Cartridge. Sorting on the MACSQuant Tyto is aerosol and droplet-free. The closed cartridge provides full operator safety and eliminates the risk of sample contamination and carry-over. The easy “plug and play” format of the instrument, combined with fully automated flow control, laser alignment and valve timing makes cell sorting accessible to any lab professional, without intensive training. In addition, all consumables are available in GMP format including GMP regulatory documentation (CoA, TSE, CoO and PIF) allowing an easy transition from a research to a clinical setting.
Features:
- Microchip-based cell sorting facilitates gentle sorting
- Sorting in a single use, disposable and fully closed cartridge system
- 3 lasers (405 nm, 488 nm, 638 nm), 8 fluorescence channels plus 2 scatter channels
- Temperature control 4°C-25°C (under standard environmental conditions)
- Flow rate up to 110 000 cells/s
Brochures
MACS GMP Tyto Consumables: GMP-compliant cell sorting with the MACSQuant Tyto System
In this product brochure, discover Miltenyl Biotec’s full range of sterile, GMP-compliant multiparametric cell sorting consumables. MACS® GMP Tyto® Consumables were specifically developed to enable cell sorting within GMP manufacturing environments using the MACSQuant® Tyto® System.
The MACSQuant Tyto Sorter - The Revolution in Cell Sorting has Begun
The MACSQuant® Tyto® is revolutionizing cell sorting. Our patented microchip-based technology opens new possibilities in basic research and medical applications with high-speed, multi-parameter flow sorting in the safety of a fully enclosed cartridge.
Sorting of rare SARS-CoV-2-specific B cells
In this application note, the MACSQuant® Tyto® is it is used to sort rare antigen-specific B cells with high purities. A combination of pre-enrichment using the REAlease CD19 MicroBead Kit and the MACSQuant Tyto Cartridge HS is used to speed up the sorting process.
Sorting and cultivation of adult neural stem cells from mice
This application note demonstrates a novel approach to purifying neural stem cells (NSCs) from the subventricular zone (SVZ) of wild type mouse brain. The tissue dissociation and cell sorting processes were optimized and automated to isolate viable single NSCs with preserved epitopes.
Complete workflow to sort highly pure and viable sub-populations of TILs from a single mouse tumor sample
This application note describes a semi-automated complete workflow to dissociate tumor tissue of a mouse model into single-cell suspensions, magnetically pre-enrich CD45+ tumor-infiltrating lymphoid cells (TILs), and subsequently sequentially sort highly pure and viable TIL sub-populations.
Gentle Sorting of PBMC Populations
Successful cell sorting via flow cytometry highly depends on the collection of viable cells. It is well established that high pressure, decompression, and shear forces negatively affect cell viability and functionality during conventional droplet sorting. Therefore, to mitigate the risk of cell damage, instrument settings, including nozzle size and pressure, have to be carefully chosen by experienced operators, who are capable of adjusting the settings accordingly. Sterility is another concern when working with traditional cell sorters that often operate in an uncontrolled, open-air surrounding. Samples are prone to contamination from both internal sources (sheath fluid, sampling fluidics, etc.) and external sources (airborne contaminants).
Isolation of CD8+ Antigen-Specific T Cells
With adoptive T cell therapies (ACTs) becoming more prevalent, the need for easy and effective isolation of antigen-specific T cells in a clinical setting is growing. The MACSQuant® Tyto™ is a benchtop cell sorter that is fully closed, sterile, and easy to use. At the heart of the system is the disposable, single-use cartridge, which allows for completely aseptic sorting conditions with no chance of cross-contamination between samples. Here we demonstrate the MACSQuant Tyto’s capacity to sort rare antigen-specific cytotoxic T cells out of fresh peripheral blood mononuclear cells (PBMCs). As a model we used PBMCs from a CMV-seropositive donor. The PBMCs contained less than 0.1% of CMV-specific T cells. CMV is a member of the herpes virus group and has a prevalence of 50–96% in human adults. Once infected, the virus persists in the body. The infection is asymptomatic in healthy individuals, but in immunocompromised patients CMV can cause severe diseases.
Cell Sorting in a Fully Closed System
The MACSQuant® Tyto® is a next generation, benchtop cell sorter equipped with 3 lasers that enable 10-parameter cell sorting. A unique feature of the instrument is the fact that the actual sorting process takes place exclusively within the single-use, disposable, and fully closed MACSQuant Tyto Cartridge, eliminating the risk of sample contamination and carry-over.
