Sorting and cultivation of adult neural stem cells from mice

3 Mar 2022

This application note demonstrates a novel approach to purifying neural stem cells (NSCs) from the subventricular zone (SVZ) of wild type mouse brain. The tissue dissociation and cell sorting processes were optimized and automated to isolate viable single NSCs with preserved epitopes.

MACSQuant® Tyto® Cell Sorter

Miltenyi Biotec B.V. & Co. KG

The MACSQuant ® Tyto ® is a next generation, benchtop cell sorter equipped with 3 lasers, which allows for high speed, 10-parameter cell sorting. A unique feature of the instrument is the fact that the actual sorting process takes place exclusively within a single-use, disposable and fully closed system: the MACSQuant Tyto Cartridge. The closed cartridge provides full operator safety and eliminates the risk of sample contamination and carry-over.

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