Accelerating cell line development with selective single-cell cloning

Cell line development workflows must balance two competing priorities: shortening the time needed to isolate production clones and identifying cells capable of delivering high recombinant protein productivity.

This whitepaper investigates a combined cloning strategy using low-viability Chinese hamster ovary (CHO) cell pools, cold-capture staining and the Beacon^® Optofluidic System. Researchers evaluated the approach across three antibody formats to determine whether early cloning and fluorescence-based enrichment could accelerate clone selection while improving titer and clone-specific productivity.

Discover how selective single-cell cloning can focus screening capacity on promising producers, reduce cell line development timelines, and support the identification of highly productive CHO clones. The study reports that using low-viability pools shortened the workflow by two weeks, while combining early sorting with cold-capture enrichment produced clones with higher productivity than the conventional workflow tested.

Resource details:

Resource type: Whitepaper
Page count: 10 pages
Read time: 15 mins