SelectScience
Join Free
ResourceDrug Discovery & Development

Overcoming drug interference in an IND-Track ADA assay

Prolytix
17 Sept 2026

Reliable anti-drug antibody (ADA) detection is essential for advancing biotherapeutics through development and regulatory review. High drug levels in patient samples can interfere with ADA assays, masking immune responses and creating false negatives that threaten assay reproducibility, delay validation, and put critical submission timelines at risk.

Explore in this resource how Prolytix overcame drug interference in an Investigational New Drug (IND)-track ADA assay using a custom approach that combined acid dissociation with the Affinity Capture Elution (ACE) method. Discover how by exposing bound ADA and reducing drug-driven interference, the assay restored sensitivity and delivered consistent, reproducible performance even in the presence of high drug levels. The result is a robust, drug-tolerant ADA workflow that supports regulatory confidence and helps keep biotherapeutic development programmes on track.

Resource details:

Resource type: Application note
Page count: 4
Read time: 5 mins

Links

ProlytixCompany website

Tags

ELISAEnzyme-linked immunosorbent assays (ELISA), also known as enzyme immunoassays (EIA), are used for the detection and quantification of proteins, peptides and antibodies in a sample. ELISA often comes in kit format, with pre-selected antibody pairs to detect specific proteins or biomarkers of diseases such as diabetes and obesity, or cardiovascular and neurological disorders. Detection is made possible with chemiluminescent, fluorescent or colorimetric substrates and detection instruments such as microplate / ELISA readers. Other kits available include: Enzyme-linked immunospot (ELISPOT) assays for the quantification of protein-producing cells and chemiluminescent immunoassays (CLIA) kits. Find the best ELISA kits and products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Pre-ClinicalProtein InteractionsProtein interactions are essential for understanding cellular processes, as proteins work together to carry out biological functions. Studying protein interactions can provide insights into disease mechanisms, drug development, and cell signaling pathways. Explore the best tools for protein interaction research in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.

Related resources