Complete workflow to sort highly pure and viable sub-populations of TILs from a single mouse tumor sample

3 Mar 2022

This application note describes a semi-automated complete workflow to dissociate tumor tissue of a mouse model into single-cell suspensions, magnetically pre-enrich CD45+ tumor-infiltrating lymphoid cells (TILs), and subsequently sequentially sort highly pure and viable TIL sub-populations.

MACSQuant® Tyto® Cell Sorter

Miltenyi Biotec B.V. & Co. KG

The MACSQuant ® Tyto ® is a next generation, benchtop cell sorter equipped with 3 lasers, which allows for high speed, 10-parameter cell sorting. A unique feature of the instrument is the fact that the actual sorting process takes place exclusively within a single-use, disposable and fully closed system: the MACSQuant Tyto Cartridge. The closed cartridge provides full operator safety and eliminates the risk of sample contamination and carry-over.

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Complete workflow to sort highly pure and viable sub-populations of TILs from a single mouse tumor sample