Cytiva Dissociation Enzyme Mixes
Cytiva’s tissue specific enzymatic dissociation kits for lung, liver, brain, and kidney offer a reliable, gentle dissociation protecting cellular transcriptional profiles. Designed for both manual and automated workflows, these tissue-specific mixes support controlled-temperature dissociation at 4°C and 37°C, delivering high cell viability, strong yield, and low aggregation.
Request a sample directly from the manufacturer.
Key Features:
- Designed for specific tissue types – Enzymes tested to an optimal level of performance for each tissue type for improved viability and yield and minimal cellular aggregation, reducing the risk of poor results in downstream applications.
- Work effectively at 4⁰C and 37⁰C** - Preliminary research has shown that cold-acting enzymatic dissociation reduces cell stress therefore minimizes cell death and improves the transcriptional profile of the single cells.
- For use with manual methods or automation – Compatible with your chosen dissociation method, including the VIA Extractor tissue disaggregator, for flexibility.
- Ready-made solution - Save the time and cost of sourcing and mixing individual components for homebrew mixes.
Brochures
Tissue enzymes testimonials
This product brochure compiles customer case studies evaluating Cytiva dissociation enzyme mixes across a wide variety of tissue types, including lung, liver, kidney, brain, stomach, and tumor samples. Discover how researchers used Dissociation Enzyme Mixes A, B, C and D to improve tissue dissociation efficiency, cell viability, and recovery for applications such as organoid culture, flow cytometry, single-cell analysis, and cell therapy research. Explore real-world performance data and user feedback highlighting workflow improvements, reduced processing times, and enhanced cell yields compared with conventional enzyme formulations.
Resource details:
Resource type: Product brochure
Page count: 32
Read time: 24 mins
Perfusion-free hepatocyte recovery using Dissociation Enzyme Mix B
Conventional hepatocyte isolation often relies on liver perfusion, which can be technically demanding and difficult to standardize across samples.
This application note from Cytiva evaluates a perfusion-free workflow using Dissociation Enzyme Mix B and automated tissue dissociation. The study assesses hepatocyte recovery, viability and morphology, with further analysis of hepatocyte and non-parenchymal cell populations using immunofluorescence staining.
Explore how an enzyme-based, perfusion-free workflow can support reproducible hepatocyte recovery and downstream liver-cell analysis.
Resource details
- Resource type: Application note
- Page count: 6 pages
- Read time: 10 mins
Perfusion-free hepatocyte enrichment protocol
Isolating viable hepatocytes from liver tissue can be challenging when perfusion is unavailable or unsuitable. This application note from Cytiva provides a step-by-step protocol for perfusion-free hepatocyte enrichment. The workflow covers tissue preparation and enzymatic dissociation, followed by sample straining, low-speed differential washes and Percoll density-gradient centrifugation.
Resource details
- Resource type: Application note
- Page count: 3 pages
- Read time: 6 mins
Cytiva's 2026 Enzyme Challenge
This flyer introduces Cytiva’s 2026 Enzyme Challenge, an opportunity for researchers to receive tissue dissociation enzyme kits and expert scientific support for challenging tissue dissociation projects. Learn how optimized enzyme formulations can help improve cell yield, viability, and reproducibility when generating single-cell suspensions from difficult tissues. Discover how selected participants can access complimentary enzyme kits and technical guidance to support manual or automated workflows and accelerate downstream single-cell analysis applications.
Resource details:
Resource type: Scientific Flyer
Page count: 1
Read time: 2 mins
Single-cell isolation of mouse liver tissue and human liver organoids using Cytiva Dissociation Enzyme Mix B
This application note evaluates the performance of Cytiva Dissociation Enzyme Mix B for generating single-cell suspensions from mouse liver tissue and human iPSC-derived liver organoids. Discover how the enzyme mix was assessed alongside perfusion methods and the VIA Extractor™ tissue disaggregator to improve cell recovery, viability, and preservation of cellular markers. Explore comparative data showing enhanced recovery of non-parenchymal liver cells, macrophages and epithelial populations, supporting applications such as single-cell sequencing, flow cytometry, and organoid characterization.
Resource details:
Resource type: Application note
Page count: 10
Read time: 10 mins
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