SelectScience
Join Free
CLINICAL24 2026
ResourceLife Sciences

Perfusion-free hepatocyte recovery using Dissociation Enzyme Mix B

Cytiva
2 Oct 2026

Conventional hepatocyte isolation often relies on liver perfusion, which can be technically demanding and difficult to standardize across samples.

This application note from Cytiva evaluates a perfusion-free workflow using Dissociation Enzyme Mix B and automated tissue dissociation. The study assesses hepatocyte recovery, viability and morphology, with further analysis of hepatocyte and non-parenchymal cell populations using immunofluorescence staining.

Explore how an enzyme-based, perfusion-free workflow can support reproducible hepatocyte recovery and downstream liver-cell analysis.

Resource details

  • Resource type: Application note
  • Page count: 6 pages
  • Read time: 10 mins

Related products

Request Quote for All Products

Cytiva Dissociation Enzyme Mixes

Cytiva

Cytiva’s tissue specific enzymatic dissociation kits for lung, liver, brain, and kidney offer a reliable, gentle dissociation protecting cellular transcriptional profiles. Designed for both manual and automated workflows, these tissue-specific mixes support controlled-temperature dissociation at 4°C and 37°C, delivering high cell viability, strong yield, and low aggregation.

(0)

Links

CytivaCompany website

Tags

Cell Lines Stem Cells and Primary CellsPrimary cell cultures, established cell lines and stem cells are vital for <i>in vitro</i> and <i>ex vivo</i> experimentation. High-quality cells, optimized for your applications, alongside optimized cell substrates, growth medium and supplements, are critical for experimental success. Explore a range of cells suitable for your applications, including isogenic cell lines, competent cells, induced pluripotent stem cell (iPSC)-derived cell lines, fungal/bacterial/mammalian cell lines, stem cells and cancer cell lines. Find the best cells for your research in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Flow Cytometry / Cell CountingFlow cytometers are used to count, sort and examine multiple characteristics of cells. Other cell analysis equipment includes image cytometers, cell counters, fluorescence-activated cell sorters (FACS), magnetic-activated cell sorters (MACS), and a range of flow cytometry assay kits. Flow cytometers can reveal information on cell viability, cell proliferation, apoptosis and cell cycle progression, as well as identify cell populations and intracellular or cell-surface molecules. Additionally, some flow cytometers, known as FACS, have an additional sorting function after analysis. Cell counters and image cytometers count live and dead cell populations and can also conduct cell proliferation assays. Find the best flow cytometers, cell counters and cell sorters in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Light MicroscopyLight microscopes or optical microscopes are used to visualize microscale objects under magnification, including cells, clinical specimens and materials. Lab equipment for light microscopy includes confocal microscopes, fluorescence microscopes, zoom and stereo microscopes. Microscope slides and imaging reagents are available for visualizing samples, as well as various microscope stages and incubators for large or temperature-sensitive samples. Find the best light microscopes in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.EnzymesCell CountingTissue DissociationHepatocytesImmunofluorescenceIn VitroIn vitro refers to experiments conducted outside living organisms, often in controlled lab environments such as petri dishes or test tubes. In vitro models are widely used in drug testing, cell biology, and disease research. Explore in vitro research tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.

Related resources