End-to-end Twist library preparation protocol for NGS workflow automation

2 Oct 2026

Streamline your NGS workflow with automated, end-to-end library preparation using the Twist Library Preparation EF Kit 2.0 and ASSIST PLUS pipetting robot. This application note presents a validated workflow for preparing up to 96 sequencing-ready libraries per run, combining precise liquid handling, active cooling and automated magnetic bead clean-up to ensure consistent, reproducible results.

Discover how to minimize hands-on time, reduce pipetting errors and improve laboratory efficiency while achieving high library yields and uniform fragment sizes. Download the application note to explore the step-by-step protocol, experimental set-up and performance results.

Resource details

  • Resource type: Application note
  • Page count: 9 pages
  • Read time: 14 mins

Twist Library Preparation EF 2.0 Kit (Enzymatic Fragmentation)

Twist Bioscience

Twist Library Preparation Enzymatic Fragmentation (EF) Kit 2.0 is built to help you achieve a more efficient sequencing pipeline and obtain more accurate Next-Generation Sequencing (NGS) results. This updated kit has new fragmentation and ligation modules, and includes the Equinox Library Prep Amp Mix. This hot-start enzyme formulation has a lower error rate* and high efficiency at low input volumes—making it ideal for samples such as FFPE, where high efficiency and yield are necessary.

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Next-Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Library PreparationLibrary preparation is a critical step in sequencing workflows, where DNA or RNA samples are converted into libraries for high-throughput, next generation sequencing. This step ensures accurate results and minimizes biases. Explore library preparation kits in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.