Perfusion-free hepatocyte enrichment protocol
2 Oct 2026Isolating viable hepatocytes from liver tissue can be challenging when perfusion is unavailable or unsuitable. This application note from Cytiva provides a step-by-step protocol for perfusion-free hepatocyte enrichment. The workflow covers tissue preparation and enzymatic dissociation, followed by sample straining, low-speed differential washes and Percoll density-gradient centrifugation.
Resource details
- Resource type: Application note
- Page count: 3 pages
- Read time: 6 mins
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Cytiva Dissociation Enzyme Mixes
CytivaCytiva’s tissue specific enzymatic dissociation kits for lung, liver, brain, and kidney offer a reliable, gentle dissociation protecting cellular transcriptional profiles. Designed for both manual and automated workflows, these tissue-specific mixes support controlled-temperature dissociation at 4°C and 37°C, delivering high cell viability, strong yield, and low aggregation.