Single-cell isolation of mouse liver tissue and human liver organoids using Cytiva Dissociation Enzyme Mix B
8 Sept 2026This application note evaluates the performance of Cytiva Dissociation Enzyme Mix B for generating single-cell suspensions from mouse liver tissue and human iPSC-derived liver organoids. Discover how the enzyme mix was assessed alongside perfusion methods and the VIA Extractor™ tissue disaggregator to improve cell recovery, viability, and preservation of cellular markers. Explore comparative data showing enhanced recovery of non-parenchymal liver cells, macrophages and epithelial populations, supporting applications such as single-cell sequencing, flow cytometry, and organoid characterization.
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Resource type: Application note
Page count: 10
Read time: 10 mins
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Cytiva Dissociation Enzyme Mixes
CytivaCytiva’s tissue specific enzymatic dissociation kits for lung, liver, brain, and kidney offer a reliable, gentle dissociation protecting cellular transcriptional profiles. Designed for both manual and automated workflows, these tissue-specific mixes support controlled-temperature dissociation at 4°C and 37°C, delivering high cell viability, strong yield, and low aggregation.