Gentle Sorting of PBMC Populations

Successful cell sorting via flow cytometry highly depends on the collection of viable cells. It is well established that high pressure, decompression, and shear forces negatively affect cell viability and functionality during conventional droplet sorting. Therefore, to mitigate the risk of cell damage, instrument settings, including nozzle size and pressure, have to be carefully chosen by experienced operators, who are capable of adjusting the settings accordingly. Sterility is another concern when working with traditional cell sorters that often operate in an uncontrolled, open-air surrounding. Samples are prone to contamination from both internal sources (sheath fluid, sampling fluidics, etc.) and external sources (airborne contaminants).