Editorial Article: Wondering which DNA probe is right for your assay?

Explore our guided navigation oligo finder with partners LGC, Biosearch Technologies

25 Apr 2022


Wondering which DNA probe is right for you?

 

 

Learn more about the DNA probes offered by LGC Biosearch Technologies

Dual-Labeled BHQ Probes

Standardize your assay. Dual-labelled BHQ Probes are traditional probes that contain a 5’ fluorophore and a 3’ Black Hole Quencher dye covalently bound to an oligo. Choose from a broad spectrum of proprietary and common market fluorophores and their corresponding Black Hole Quencher dye to obtain high-quality qPCR data.

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Strengthen your assay with shorter probes specifically designed to enhance specificity and improve mismatch discrimination through the use of modified C and T nucleotides. Reduce assay cost and improve data quality by redesigning MGB probe assays with BHQplus technology.

 
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Power your assay when longer probes are needed by substantially reducing background interference and increasing the signal-to-noise ratio with double-quenched BHQ probes utilizing the power of both an internal nova quencher and a 3’ BHQ quencher.

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LNA probes

Improve assay sensitivity and specificity with Locked Nucleic Acid oligonucleotides (LNA™ oligos). LNAs are modified RNA bases in which the ribose is “locked” with a methylene bridge, connecting the 2' oxygen atom to the 4' carbon atom, fixing it in the C3'-endo conformation.

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Choose premier KASP™ on Demand (KOD) service for design and delivery of your KASP assay, plus full validation and optimization of your assay.

 
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Choose standard KASP™ by Design (KBD) service for design and delivery of a KASP assay to your SNP or indel of interest. Assay design (primer selection) is carried out using proprietary Kraken™ software system.

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A dual-labelled probe that relies on a hairpin conformation to heighten assay specificity. This property allows Beacons to discriminate mismatches as specific as a single nucleotide polymorphism (SNP). Beacons generate fluorescence through hybridization and under non-hydrolytic conditions allowing post application PCR melt curve analyses.

 
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A dual-labelled probe that combines a hairpin structure and a PCR primer element in a single oligo, allowing for target detection through a unimolecular mechanism. Mismatched hybrids are less stable than the reformed stem region. Therefore, Scorpions Primers only produce signal when the probe region hybridizes to a perfect-match target sequence within the primer extension product.

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