DRAQ5

In this whitepaper, Biostatus Limited examines sample preparation and the concerns over quality assurance of cell delivery for downstream analysis. This whitepaper highlights that sample preparation will improve quality and validity of the molecular analysis when performed to a high standard.

DRAQ5™ has been used widely in high content screening and related drug discovery assays for more than a decade. Recent papers give a clear overview of the different intracellular features one can render with DRAQ5™ staining.

DRAQ5™ and DRAQ7™ dyes provide accurate fluorescent labelling and assist in distinguishing cell status for single cell transcriptomics and genomic applications. One concern regarding the use of these DNA binding dyes is any unwanted effects on the first strand cDNA synthesis. However, studies in this white paper have demonstrated that DRAQ5™ and DRAQ7™ do not interfere with the synthesis process, indicating an accurate experimental outcome.

The cell cycle is a series of events that occur when a cell divides and replicates into two daughter cells. Cell cycle regulation depends upon processes that are pivotal to cell survival. During the cell cycle the quantity of DNA increases from 2N (G1 phase) to 4N (G2 phase) via the S (synthesis) phase. Perturbation of the cell cycle can lead, for example, to cell cycle arrest or uncontrolled cell division, as in cancer, while cells with DNA below 2N are typically in apoptosis, this is the, so-called, sub-G1/G0 peak. The quantity of DNA in each nucleated cell in a population can be measured by labeling the nuclear DNA with a fluorescing DNA dye, most commonly propidium iodide (PI). As a live-cell permeant DNA probe DRAQ5™ can be applied directly to complex samples such as blood or bone marrow or cultured cells, as the last step prior to analysis and without any washing steps.

In apoptosis an ordered series of events leads to destruction of a single cell. The changes that occur can be assayed through DNA fragmentation, caspase activity (using permeant substrates e.g. FLICA™ probes), mitochondrial membrane potential (e.g. using TMRM), cytochrome C release, plasma membrane inversion (using Annexin V) and permeabilization being common choices. This application note demonstrates the use of APOPTRAK™ in the analysis of far-red fluorescent dye reporting cell integrity.

This application note demonstrates a number of applications of Fluorescein-/GFP-compatible dyes, in particular DRAQ5 and CyTRAK Orange. The particular spectral and chemical features of DRAQ5 and CyTRAK Orange mean they are compatible with aqueous media and fixatives. Conveniently, they are supplied in aqueous solution and can be applied as the final step in a sample preparation. They have become standard reagents for immunofluorescence microscopy, flow cytometry and high content imaging.

BioStatus has become only the third company to receive a coveted SelectScience Platinum Seal of Quality for its DRAQ5. The DNA staining dye is also the very first consumable to win the highest level of Seal of Quality - a reflection of how much it is loved by scientists around the world. Seals of Quality are only presented to products which attract the required number of positive reviews from scientists working in labs around the world. In this video, Roy Edward, sales and marketing director at BioStatus, outlines just how much it means to win a SelectScience Seal of Quality.

DRAQ7™ is a new far-red fluorescent DNA dye that only stains the nuclei in dead and permeabilized cells. DRAQ7™ has many applications in imaging, cytometry and screening and is highly compatible with existing protocols across a wide range of instrumentation platforms. Watch this video to learn how DRAQ7™ can be used to monitor cell health, cell by cell.

DRAQ5™ is a live cell-permeant, far-red fluorescent DNA dye from BioStatus. Learn how DRAQ5™ enables multi-color analysis of complicated samples, via flow cytometry and cell based imaging.

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Discover the results of a recent Pfizer study which demonstrate a new in vitro toxicology application for DRAQ5.

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