APOPTRAK™ in FC-Based Apoptosis Assays: Live Cell Marking and Cell Death Scoring

In apoptosis an ordered series of events leads to destruction of a single cell. The changes that occur can be assayed through DNA fragmentation, caspase activity (using permeant substrates e.g. FLICA™ probes), mitochondrial membrane potential (e.g. using TMRM), cytochrome C release, plasma membrane inversion (using Annexin V) and permeabilization being common choices. This application note demonstrates the use of APOPTRAK™ in the analysis of far-red fluorescent dye reporting cell integrity.