Next Generation Sequencing Products & Reviews

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Next-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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SureSelect Cancer CGP Assay ​

Agilent Technologies

SureSelect Cancer CGP assay offers comprehensive genomic profiling for solid tumors. This pan-cancer NGS panel enables detection of somatic variants, including single nucleotide variants (SNVs), copy number variants (CNVs), insertions/deletions (indels), translocations (TLs), gene fusions, and the immuno-oncology biomarkers TMB (tumor mutational burden) and MSI (microsatellite instability).

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SureSelect DNA Capture Arrays

Agilent Technologies

Designed for smaller studies, the SureSelect DNA Capture Arrays complement Agilent's in-solution SureSelect Target Enrichment System, which is designed for medium to large-scale NGS studies of tens through thousands of samples, including automated high-throughput workflows.

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SureSelect Custom RNA

Agilent Technologies

Agilent’s market leading SureSelect platform provides a complete portfolio of catalog and custom products, providing the flexibility you need from discovery to follow-up studies. Agilent’s custom solutions offer the only available method for target enrichment of RNA, allowing you to target your regions of interest using our free web portal SureDesign. Custom solutions are available for DNA and RNA target enrichment. • The only…

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Microlab® NIMBUS NGS workstation

Hamilton Company

Hamilton’s Microlab® NIMBUS NGS workstation provides a completely automated and affordable solution for emPCR emulsion breaking, enrichment and sequencing primer hybridization for the Roche GS Junior System. The fully tested and validated NIMBUS NGS workstation features an integrated REM e system and provides convenient walk-away automation solution to minimize laborious manual process and reduce human errors.

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VeriFi® Hot Start Mix

PCR Biosystems

VeriFi® Hot Start Mix is a versatile and robust 2x proofreading ready-mix with AptaLock™ hot start technology for highly precise PCR. Improved DNA binding and increased processivity give significant improvements in speed, yield and sensitivity while also increasing PCR success rates of long and challenging templates.

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VeriFi® Hot Start Mix Red

PCR Biosystems

VeriFi® Hot Start Mix Red is a versatile and robust 2x proofreading ready-mix with AptaLock™ hot start technology for highly precise PCR. Improved DNA binding and increased processivity give significant improvements in speed, yield and sensitivity while also increasing PCR success rates of long and challenging templates. The mix includes a convenient red dye for direct gel loading.

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VeriFi® Hot Start Polymerase

PCR Biosystems

VeriFi® Hot Start Polymerase is a versatile and robust proofreading enzyme with AptaLock™ hot start technology for highly precise PCR. Improved DNA binding and increased processivity give significant improvements in speed, yield and sensitivity while also increasing PCR success rates of long and challenging templates.

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NGSBIO Library Quant Kit Blue for Illumina

PCR Biosystems

The NGSBIO Library Quant Kit Blue contains all the components required for accurate and sensitive quantification of libraries prepared for Illumina NGS systems. The kit uses an easy-to-see blue qPCR mix to specifically quantify adapter-ligated DNA molecules, ensuring optimal cluster densities for improved sequencing efficiency and quality of data.

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VeriFi® Library Amplification Mix

PCR Biosystems

VeriFi® Library Amplification Mix is ideal for NGS library amplification workflows and challenging PCRs. Combining a powerful and robust proofreading enzyme, greatly reduced GC-dependent bias, and AptaLock™ hot start technology, this mix enables precise PCR, regardless the target you are sequencing.

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Twist Fast Hyb & Wash Kit

Twist Bioscience

Twist’s Fast Hybridization and Wash Kit is a revolutionary new system with the flexibility to both save you time and improve the performance of your targeted sequencing efforts. It accommodates your samples, your schedule, and your performance needs: accelerate hybridization to as little as 15 minutes, or adjust the hybridization time to improve coverage for all targeted sequencing applications, including whole exome, custom…

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Twist Universal Blockers

Twist Bioscience

Twist Universal Blockers enhance the specificity of next-generation sequencing (NGS) target enrichment by preventing non-specific hybridization between adapter sequences, thereby improving on-target capture and reducing sequencing costs.

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Twist Human RefSeq Panel

Twist Bioscience

The superior performance of Twist Human Core Exome provides the optimal solution for sequencing of protein coding genes. The Twist Human Core Exome however focuses only on the most accurate curated subset—CCDS database. Twist now offers the Twist Human RefSeq Panel, which is designed to expand the content of the Twist Human Core Exome. When combining the two panels, greater than 99% of protein coding genes are covered.

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Twist Library Preparation MF Kit (Mechanical Fragmentation)

Twist Bioscience

Twist Library Preparation Kit (Mechanical Fragmentation) is designed for use with gDNA that has already been sheared mechanically. It combines the enzymes and reagents for end repair, dA-tailing, and adapter ligation into a single reaction: Accommodates a wide range of DNA input types, enables high-quality sequencing of low-quality samples and minimizes start / stop site artifacts

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Twist Library Preparation EF Kit (Enzymatic Fragmentation)

Twist Bioscience

Twist Library Preparation EF Kit (Enzymatic Fragmentation) incorporates the reagents and enzymes needed for DNA fragmentation with those for end repair, dA-tailing, and adapter ligation into a single reaction:  Ideal for automated, high-throughput library preparation, produces tunable, reproducible fragment sizes and minimizes sequence bias, maximizes coverage depth.

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