ExoBrite™ Annexin EV Staining Kits
Fluorescent Annexin V conjugates that are optimized for bright and low background staining of extracellular vesicles for flow cytometry.
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ExoBrite™ Annexin EV Staining Kits were designed to overcome some of the challenges of EV detection, particularly in flow cytometry. ExoBrite™ Annexin EV Stains bind to molecules in the EV membrane for bright, specific staining, with little to no background.
Features
- Optimally formulated Annexin V conjugates for staining purified EVs
- Broad compatibility with EVs isolated from all sources tested
- Designed for detection by flow cytometry
- Bright signal and low background
- Compatible with antibody co-staining
- Available in 4 colors
ExoBrite™ Annexin EV Stains are uniquely formulated conjugates of Annexin V, a 35-36 kDA calcium-dependent phospholipid-binding protein with high affinity for phosphatidyleserine (PS). Annexin V conjugates have been used to detect EVs due to the presence of PS on most EV membranes.
ExoBrite™ Annexin EV Stains were designed to overcome some of the challenges of EV detection, particularly in flow cytometry. For example, lipophilic membrane dyes commonly used to stain EVs can form aggregates of a similar size as exosomes or EVs, thus confounding analysis. Conversely, ExoBrite™ Annexin EV Stains are specially formulated to minimize aggregation in flow cytometry, allowing EVs to be identified with bright staining with minimal background. In addition, ExoBrite™ Annexin EV Stains were designed to offer broad coverage of EVs isolated from different sources. We tested EVs derived from 9 cell lines and ExoBrite™ Annexin EV Stains showed strong staining for all of them.
EVs are often labeled with fluorescent antibodies targeting one or more of the tetraspanin proteins CD9, CD63, and CD81. ExoBrite™ Annexin staining can be combined with antibody staining, for multi-parameter analysis.
ExoBrite™ 410/450 Annexin EV Staining Kit, 500 labelings #30119
ExoBrite™ 410/450 Annexin EV Staining Kit, 100 labelings #30119-T
ExoBrite™ 490/515 Annexin EV Staining Kit, 500 labelings #30120
ExoBrite™ 490/515 Annexin EV Staining Kit, 100 labelings #30120-T
ExoBrite™ 560/585 Annexin EV Staining Kit, 500 labelings #30121
ExoBrite™ 560/585 Annexin EV Staining Kit, 100 labelings #30121-T
ExoBrite™ 650/665 Annexin EV Staining Kit, 500 labelings #30122
ExoBrite™ 650/665 Annexin EV Staining Kit, 100 labelings #30122-T
