Member since: 2019
Organization: Leibniz-Forschungsinstitut für Molekulare Pharmakologie
Great system with broad detection portfolio. Ideal for assay development and screening!
Application Area: HTS for drugs (70.000 compounds) and gene function (RNAi & CRISPR-Cas)
"This detection system nicely bridges the gap between a plate reader and imaging systems like automated microscopes. It combines multi parameter screening (all kinds of fluorescence detection) with the features of image based screening (cytometric measurements) in one machine. Coupling kinetic measurements over long time periods to injection of reagents at the most effective time points within the reader. Ideal system to be used in assay optimisation and in screening. Nice graphical interface for assay set up and a lot of nice analysis features like confluence or viability measurements."
Spark Cyto builds on the foundation of the original Spark platform, combining the flexibility of a high-end multimode plate reader with whole well imaging and comprehensive environmental control to allow multiparametric measurements for cell-based applications.
This dynamic instrument uses real time data acquisition and analysis to deliver meaningful insights faster than ever before, ensuring that no key event is ever missed. It combines live cell imaging with industry-leading detection technologies, allowing qualitative and quantitative information to be integrated into unique multiparameter data sets. Top-of-the-range camera components and patent-pending LED autofocus technology offer three magnification levels and four acquisition channels (for fluorescence and bright field imaging), allowing the investigation of entire cell populations, and enabling the whole well area of a 96- or 384-well microplate to be recorded with just one image, without tiling or distortion.
Spark Cyto is designed to handle a broad range of common cytometry applications, offering a new level of experimental control without compromising on ease of use and convenience. Powerful SparkControl™ and Image Analyzer™ software give the operator complete control of all the parameters associated with their experiment, with five predefined methods to offer straightforward image acquisition and analysis for common cytometry applications. These are complemented by additional features – such as ‘user-defined’ parameters and Real Time Experimental Control (REC™) to enable kinetic experiments to be performed automatically – to deliver multiplexed live cell data from a single instrument, unlocking new possibilities for cell-based research and giving increased confidence in the statistical relevance of the results.
Spark Cyto is available in four specialized configurations with different detection modalities, each fully equipped for real-time live cell imaging cytometry.
Spark Cyto is for Research Use Only.