DAWN® by Wyatt Technology Corp.

Manufacturer Wyatt Technology Corp.  |   Model: WH2  |  Available Worldwide
4.5
/
5.0
  |  112 reviews

The DAWN is the premier Multi-Angle static Light Scattering (MALS) detector for absolute characterization of the molar mass and size of macromolecules and nanoparticles in solution, offering the highest sensitivity and widest range of molecular weight, size, concentration and optional features.


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DAWN®
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Average Rating: 4.5
112 Scientists have reviewed this product

4 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2016

  • Organization: University of Alberta



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    5 out of 5
A must have in modern biochemical laboratories.
Rating: 4.3

  • Application Area: Oligomerization and arrangement of biomolecules

"DAWN is an easy to use multi angle light scattering detector. It allows you to analyze the molecular size with in-line with SEC and thus, we can visualize changes in size easily on protein-protein and protein ligand interactions."

Review date: 07 Jun 2019 | DAWN®
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: MCPS



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Most versitile SEC detector on the market - great product.
Rating: 5.0

  • Application Area:Polymer analysis

"I have been using MALS since the 1980's - Wyatt's have always been the best value. This latest is very user-friendly. The combined information from the MALS and the viscometry detector can't be beaten. Branched co-polymers are no problem; great for pH."

Review date: 26 Mar 2019 | DAWN®
  • Status:

    Reviewer

  • Member since: 2018

  • Organization: Biological E Limited



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Without this lot quality control checks for the product would have not possible.
Rating: 5.0

  • Application Area:Biomolecules size and concentration determination

"This is the only technology available for absolute molecular size determination with ease of use. It is highly reproducible and recommended for polysaccharide and conjugate vaccine manufacturers."

Review date: 18 Feb 2019 | DAWN®
  • Status:

    Advanced Reviewer

  • Member since: 2016

  • Organization: University of Saskatchewan



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Easy to use to determine protein oligomerization state.
Rating: 5.0

  • Application Area:Protein characterization

"Easy to use to determine protein oligomerization state with connection to FPLC it is possible to check fraction on SDS-PAGE gel."

Review date: 20 Dec 2018 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Alberta



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Amazing results and easy analysis.
Rating: 4.3

  • Application Area:Protein complexes, protein-protein, protein-lipid interactions.

"It is very effective in measurement of the molecular mass of a protein in-line to SEC. We are able to differentiate small changes in size. It is easy to use instrument."

Review date: 11 Dec 2018 | DAWN®
  • Status:

    Reviewer

  • Member since: 2018

  • Organization: Christian-Albrechts Universität zu Kiel



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
In some experiments great results, want to explore more.
Rating: 4.7

  • Application Area:Analysis of fucoidans (sulfated polysaccharide) from brown algae

"Use of MALS coupled with RI detector for the analysis of polysaccharide is outstanding if the separation technique is sufficient. Service after sales is simply the best. If you want to characterize large biomolecules, it is one of the best options."

Review date: 21 Jun 2018 | DAWN®
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: University of Manitoba



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
The instrument is excellent and a useful addition to any protein Biochemistry lab.
Rating: 4.7

  • Application Area:Protein and protein complex molecular weight determination

"The instrument is very easy to use and the results are reproducible provided you take care to ensure that you have your sample in exactly the same buffer as that used for the MALS experiment. It enables a faster initial estimation of protein complex stoichiometries than is available by Analytical Ultracentrifugation and it is extremely useful for estimating the oligomeric state of membrane proteins by separating the protein and detergent components. We now routinely use the instrument to assess the oligomeric state and purity of all proteins we work with and we have incorporated it into all our protein purification SOPs."

Review date: 31 Jul 2017 | DAWN®
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: UMSA



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
It is a great detector
Rating: 4.7

  • Application Area:particles and macromoleculs

"This detector couple with dRI detector are a very useful tool for characterizations of macro molecules and particles as well."

Review date: 22 Jul 2017 | DAWN®
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: BioMarin Pharmaceuticals



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
This equipment provides high quality reproducible data with minimal effort.
Rating: 5.0

  • Application Area:Charecterization of drug products

"DAWN HELEOS II is one of the best equipment for size , molecular mass and number density characterization. Along with SEC/FFF this proves to be a highly advanced technology for comparability and analytical characterization of different biological molecules."

Review date: 21 Jul 2017 | DAWN®
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Boise State University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Provides rapid results integral to research
Rating: 4.7

  • Application Area:analyze novel linear and branched polymers

"Very easy to use. Using only THF, the instrument is very reproducible and is requires minimal upkeep. Results match other techniques reasonably."

Review date: 20 Jul 2017 | DAWN®


  • Status:

    Reviewer

  • Member since: 2017

  • Organization: HHNI@MB@B Yale University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great instrument! We use it on a regular bases during our purification process.
Rating: 5.0

  • Application Area:Structural Biology

"We have been very pleased with the instruments performance and ease of use. It has given us valuable information on the oligomeric states of the proteins and protein nucleic acid complexes that we are trying to crystallize."

Review date: 20 Jul 2017 | DAWN®
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Lyondellbasell



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Works good but would prefer something more simple.
Rating: 4.0

  • Application Area:Analyze absolute molecular weight distribution of polyethylene samples in a high temperature GPC sys

"The 18 angle LS detector works really well and I am overall pleased with its functionality. However, for this application 18 angles is definitely overkill. It slows down our GPCone software and leaves more opportunities for malfunctions. So far we have not had to make any repairs, but I have heard that making repairs on this system can be very difficult. It would be nice if they made a simpler, easy to repair, 3-6 angle detector for this type of application."

Review date: 20 Jul 2017 | DAWN®
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Codiak Biosciences



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Fantastic instrument, best in class!
Rating: 4.7

  • Application Area:Particle and protein size characterization

"Great instrument. Solid robust performance. Instrument is fairly easy to use after training and easy to implement into workflows."

Review date: 20 Jul 2017 | DAWN®
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: NIH



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
The results are great and reliable for quality control.
Rating: 5.0

  • Application Area:analyze protein and protein conjugates

"We have implemented this method as the quality control for our protein conjugates. the results are included in FDA approval submission. The conjugates products are used as vaccine in a clinical trial."

Review date: 20 Jul 2017 | DAWN®
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Celldex Inc



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Great instrument, excellent service!
Rating: 4.0

  • Application Area:Protein Characterisation

"The Wyatt staff has been extremely helpful in assisting us in our applications. "

Review date: 20 Jul 2017 | DAWN®
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Changchun Institute of Applied Chemistry, Chinese Academy of Sciences



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great machine, one must have it in a lab for characterizing polymers.
Rating: 5.0

  • Application Area:Analysis of polymers, liposomes, particles, etc.

"It is a fantastic MALS detector, easy to use and vary reliable. I have used/seen this detector in many labs, always happy to work with this MALS detector."

Review date: 20 Jul 2017 | DAWN®
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: PPG



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Wyatt is a great company and makes a great product.
Rating: 5.0

  • Application Area:Analyzing particles and polymers

"The results that we achieve are very high quality and the instrument is easy to use."

Review date: 20 Jul 2017 | DAWN®
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: XX



  • Ease of use
    4 out of 5
    After sales service
    3 out of 5
    Value for money
    5 out of 5
Great instrument
Rating: 4.0

  • Application Area:Polymer analysis

"Great instrument. Much better than previous instrument that I've used."

Review date: 20 Jul 2017 | DAWN®
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Takeda California



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great results
Rating: 5.0

  • Application Area:Analyze protein samples

"The MALS detector produced critical data that allowed for routine QC of the target protein for our drug discovery program"

Review date: 15 Apr 2017 | DAWN®
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Abbvie



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Absolutely cannot do without
Rating: 5.0

  • Application Area:Biologics: antigen and antibody characterization.

"If you are still uncertain about the limitations of SEC, well it's time for you to learn about them. There is no protein biophysics without knowledge of molecular mass in solution. That is such a critical and central piece of information to have on your proteins and no instrument out there will give you a better estimate of it than the Heleos. Wyatt customer since 2008, couldn't be happier about the analytical power they provide."

Review date: 01 Apr 2017 | DAWN®
  • Status:

    Expert Reviewer

  • Member since: 2015

  • Organization: Ajinomoto Althea



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
One of the best purchases we've ever made for our lab!
Rating: 5.0

  • Application Area:Measure molecular weight of samples downstream of SEC

"Without the DAWN HELEOS II, we'd be skeptical of our molecular weight measurements by SEC and column calibration alone. The DAWN allows us to rest assured that we are getting reliable data. Wyatt has also been exceptional with our unusual application requests."

Review date: 20 Dec 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Howard University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
It is the best and easiest way to do the characterization.
Rating: 5.0

  • Application Area:PEG-Antibody conjugates characterization

"The instrument allows the researcher to determine the degree of PEGylation of antibody."

Review date: 24 Oct 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2014

  • Organization: SYNPO



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
The instruments are the essential tools for every polymer laboratory.
Rating: 5.0

  • Application Area:Synthetic polymers

"Great instruments for the characterization of molecular structure of polymers. Especially useful for the identification and characterization of degree of branching. I appreciate the reproduciblity of obtained results."

Review date: 26 Jul 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Canadian Grain Commission



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Excellent reliability and service
Rating: 5.0

  • Application Area:Determine molecular weight of polysaccarides

"Once the product is set up and calibrated it is very easy to use, even with large amounts of time in between not being used. The instrument is very reliable and accurate. Once it has been calibrated my control standard is very reproducible. The phone help is excellent, there is no wait time and they will do everything necessary to answer your questions or fix your instrument. When I send my instrument in for yearly service it is completed in a reasonable time frame and any work done is fixed and report to me."

Review date: 24 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Manitoba



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
An absolute must for today’s protein biochemist
Rating: 5.0

  • Application Area:Protein sizing

"This instrument is perfect for understanding protein/detergent complexes and ascertaining the stoichiometry of protein/protein complexes. Wyatt also offer an excellent training course which is backed up by an excellent support team who are always on hand to help out."

Review date: 24 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Boehringer Ingelheim



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Can't beat light scattering for first principle mass detection especially with glycoproteins.
Rating: 4.0

  • Application Area:Protein and glycoprotein characterization

"The DAWN HELEOS is a technologically advanced and well-designed light scattering system. When a glycoprotein won't "fly" in mass spec, we use the HELEOS in triple detector mode to characterize the amount of glycosylation. As a first principle method returning mass, it is useful for any all proteins and tight complexes of macromolecules. The Wyatt flow cell and software are an excellent way to implement this method. "

Review date: 24 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Laboratory of Polymer Chemistry, Department of Chemistry, University of Helsinki



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Possibly the best commercial MALS and RI detectors currently available on market.
Rating: 5.0

  • Application Area:polymers in aqueous solutions

"HELEOS is a highly professional and easy to use detector of our AF4 device. The possibility to use it together with a refractive index detector Optilab rEX in a batch/stand-alone mode is an excellent feature. It is really easy to convert HELEOS and Optilab rEX from detectors into high quality scientific devices. We work with thermally responsive polymers and HELEOS with precise temperature control is just great. Importantly, the results are obtained practically instantaneously, especially if compared with goniometer-type instruments. We also receive very quick and professional help from Wyatt services: we are specialists in light scattering but we ask for help in respect to usage of AF4 and software (Astra). For potential customers: LS is a highly informative but difficult method. Wyatt products can do much more than an average user can imagine. In my opinion, a physicist specialized in polymers/particles in solutions should run AF4, HELEOS and Optilab rEX. It is not enough to run an experiment. One should know physical properties of polymers in order to design the experiment correctly. Only then the true value of Wyatt instruments can be appreciated."

Review date: 24 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: NIFDC



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
a fantastic machine, hope to give a more economical price.
Rating: 4.3

  • Application Area:pharmaceutical research

"The instrument is very useful on the application of pharmaceutical researches, especially large molecular biological products. It has high precision and sensitivity. Presently there is no substitutional production at China market. It has friendly media and is relatively easy to learn how to use. It will be better if the company provide a ready-to-use instruction and same examples of how to utilize the instrument. "

Review date: 24 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: LUH Hannover, Germany



  • Ease of use
    3 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
After some training, very good results
Rating: 4.0

  • Application Area:Protein and complexes analysis

"Excellent contacts with application lab in Germany. The scientists are helpful and responsive and very knowledgeable. Instrument needs expert handling (as many other instruments, though)."

Review date: 24 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Missouri-Columbia



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Can't live without this instrument use it almost monthly
Rating: 5.0

  • Application Area:Analytical analysis on protein mass, lipids, and fractionation of bulk materials

"Very easy to use especially go through the training class on site at Santa Barbara Great customer support both online and by phone, detailed instructions on all trouble shooting materials Provide great and precise molecular weight based on both size and shape Instrument is very durable and seldom need repair Major pieces of equipment for my project and for publication "

Review date: 24 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Johnson & Johnson



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
The best detectors around, and the best customer service to boot!
Rating: 5.0

  • Application Area:Analysis of synthetic polymers

"All of our Wyatt instruments perform very well. I have used detectors from a number of manufactures, and Wyatt has consistently been of the highest quality. Not only that, but the Wyatt customer service is the best. I wouldn't purchase anything else."

Review date: 24 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: American University of Sharjah



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
We need our particle size to complete our research, without it we would not be able to characterize our nanocarriers
Rating: 4.3

  • Application Area:analyze nano-particle sizes.

"The product is effective and we were able to find the particle sizes for liposomes and micelles as needed. We also had a tech. support visitor, visit our campus and help us understand all the functions of the equipment. "

Review date: 24 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: INM - Leibniz Institute for New Materials



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
good results, useful and robust instrument
Rating: 4.0

  • Application Area:detector for Field-Flow Fractionation, nanoparticle analysis

"Instrument can be used by many researchers without major problems. MALS and DLS theory and the opportunities of the instrument could be presented in a more convenient way. Also, the main issues like high baseline noise and contamination could be summarized in a separate application note/ white paper (instead of reading the whole manual). I would appreciate more tutorial videos instead of written documents. "

Review date: 24 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: McGill University



  • Ease of use
    3 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
good instrument but a more detailed protocols for data analysis would be appreciated
Rating: 3.7

  • Application Area:look at oligomerization state of purified proteins

"We obtained good results for the analysis of soluble proteins. However, for the protein conjugate analysis, I find very little documentation about the practical aspect of that kind of analysis. "

Review date: 24 Jun 2016 | DAWN®

Manufacturer's Response

Dear Dr. Sauve, Thank you for your valuable feedback. We’re glad to hear that your light scattering instrument is giving you good results. During the past few years we’ve been developing multiple guides and instructions on how to set up and analyze light scattering experiments, and we also have a detailed technical note on protein conjugate analysis. Please email support@wyatt.com, and we’ll be delighted to email our technical note to you! Best regards, Wyatt Technology Corporation
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Université du Québec à Chicoutimi



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
This instrument was a great upgrade in our lab and in our undergraduate chemistry program!
Rating: 4.7

  • Application Area:Undergraduate teaching (polysaccharides, proteins)

"We appreciate working with the instrument and our student learn a lot on macromolecules with that. The after sales care has always been perfect. The only thing that we don't fully appreciate is the software Astra 5, it's not really easy for a new person to understand it quickly. "

Review date: 24 Jun 2016 | DAWN®

Manufacturer's Response

Dear Dr. Perron, Thank you for your valuable feedback on our software. We have a newer software version (ASTRA 6) that was out a few years ago, which is much easier to use. We invite you to contact support@wyatt.com to share details on how we can improve our software – maybe the newer software already takes care of all your needs. We’ll be also glad to send you some guides and instructions on how to set up SEC-MALS experiments and perform the various analyses in ASTRA. Best regards, Wyatt Technology Corporation
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Verily



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Really solid instrument that produces useful data
Rating: 4.3

  • Application Area:Analyze protein, polymer, and nanoparticles in solution

"This is an excellent MALS/DLS instrument that we use regularly."

Review date: 24 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Oregon State University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Excellent results and tremendous customer service even years after purchase. Technical support on fundamentals (and not only equipment trouble-shooting) is excellent.
Rating: 5.0

  • Application Area:Water soluble polymer and protein molecular weight characterization

"We have worked with Wyatt Technology multi-angle laser light scattering (MALLS) equipment for over 15 years. We have used this exclusively for aqueous polymer and biological fluid (equine synovial fluid) characterization in static and GPC-MALLS modes. This has been used by a wide variety of people at various levels of technological acumen. The fundamentals of light scattering may be difficult to learn, but the use of the Wyatt equipment to get good data is relatively easy for a careful experimentalist. I have sent 4 students to the Wyatt Light Scattering University (LSU) and this has been extremely beneficial. The Wyatt technical support has always been outstanding, not only on equipment operation, but also on helping to optimize and understand difficult experiments. Frankly, I would never consider buying polymer molecular characterization equipment from any other company. "

Review date: 24 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Ghent University



  • Ease of use
    3 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
Very handy machine to have in the lab
Rating: 3.3

  • Application Area:determination of oligomeric state and detergent contribution of membrane proteins

"Our MALS system from WYATT is coupled with SEC. The obtained results are high quality and reproducible, but preparation of the machine for run takes a lot of consumables. By a lot I mean ~5 column volumes, to have stable readout for light scattering and refractive index detectors."

Review date: 24 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2008

  • Organization: Cabot Microelectronics Corporation



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Definitely the best light-scattering detector out there.
Rating: 4.7

  • Application Area:molecular weight determination of various aqueous polymers

"The Heleos II detector is durable and easy to use if one has previous experience with light-scattering instruments. Equilibration of the instrument is fast and it is very easy to switch between solvents. The ability of the instrument to revert to stand-by (idle) mode is particularly useful for running a sequence overnight when no one is around to oversee the experimental run. Maintenance is also easy. "

Review date: 24 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: The Goodyear Tire & Rubber Co.



  • Ease of use
    4 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
Good, reliable data in a reasonable time
Rating: 3.7

  • Application Area:Solution Polymer (Elastomers) Mw / Mnand PDI

"The instrumentation and software work very well for our process and finished product needs. Very little maintenance needed and only occasional calibration checks required. "

Review date: 24 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2006

  • Organization: Alfatech SpA



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
It has changed my life
Rating: 5.0

  • Application Area:absolute molecular weight of polysaccharides

"I'm using such products since more than 25 years, the results are always very robust and reproducible, the software allows to get a lot of useful information on size and shape of the molecule."

Review date: 24 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Dow Chemical Company



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
It is great instrument.
Rating: 5.0

  • Application Area:Polymer molecular weight and molecular architecture characterization

"The instrument is very reliable and working very well. It is a critical instrument for absolute molecular weight determination of polymer. Software is user-friendly."

Review date: 24 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Cambridge



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
A good source of a variety of information!
Rating: 4.7

  • Application Area:Novel polymer characterisation

"The HELEOS provides us with good reproducible results, I think the main issue we have is with our lack of suitable SEC columns for our wide variety of synthetic polymers! But this is where the real utility has been shown for the instrument, as we regularly run batch mode Zimm plot experiments to counteract this."

Review date: 24 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: SOLVE Research & Consultancy AB



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
A central part of our instrumentation
Rating: 4.7

  • Application Area:analysis of biopolymers, proteins and nanoparticles

"Highly sensitive detector with very good s/n ratio"

Review date: 24 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Small Biotech



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Really useful, great for following protein aggregation
Rating: 4.3

  • Application Area:Protein Molecular Weight Determination

"Really good instrument, only limited by the quality of our HPLC. Works even for relatively small proteins. Mostly used for working out what is going on with complex samples. Used for identification of multimers."

Review date: 24 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Oxford



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    5 out of 5
Excellent results, no matter what the shape of the protein we get a spot-on MW value
Rating: 4.3

  • Application Area:Protein characterisation

"Excellent results, no matter what the shape of the protein we get a spot-on MW value. One of the most popular techniques in our facility. Researchers now trust the results so much that they can identify ligands bound to proteins that they were not expecting. The courses at Santa Barbara were excellent. Would recommend going as soon as you got the instrument. Because it is so heavily used I would appreciate some sort of maintenance programme just for the confidence that we could get it repaired if anything did go wrong. "

Review date: 24 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: Senmin Iternational



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Money well spend we better understand characteristics of our polymers more than ever before.
Rating: 5.0

  • Application Area:Analysis of CMC and Polyacrylamides using Wyatt triple detectors,

"Since it was the first time for me to do polymer characterization using the Wyatt Optilab (TreX), Dawn Heleos and Viscostar the three days training I attended at Santa-Babra helped a lot in understanding the use of the detectors and better processing of results using the Astra Software. On our CMC samples we currently running we able to get accurate, reproducible and reliable results. Although we still experiencing some difficulties with our Polyacrylamides samples the help we getting from the Wyatt support team have been very helpful and we confident that all our problems will be resolved soon."

Review date: 24 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: London School of Hygiene and Tropical Medicine



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Top shelf lab equipment: fast results with small amount of sample, very reproducible and reliable method.
Rating: 5.0

  • Application Area:vaccine analysis, protein conjugates

"We use SEC MALS dRI that gives us excellent data set on sample quality and heterogeneity. The multi-angle light scattering detector allows us to detect larger aggregates and segregate them out of vaccine solution. Additionally, we obtain hydrodynamic radious calculation as well as molecular weight of a protein. "

Review date: 24 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Currenta GmbH & Co. OHG



  • Ease of use
    1 out of 5
    After sales service
    2 out of 5
    Value for money
    3 out of 5
Good hardware, but the software could be improved.
Rating: 2.0

  • Application Area:SEC with MALLS, mostly for analysis of synth. rubber, structure & branching analysis

"The technology in the instrument DAWN HELEOS II is properly chose. The signals are clear and the measurement is sensitive. In the other hand the software isn't customer friendly. First the Option for a properly SEC Quality Management doesn’t exist. The creation of a calibration is time/consuming. Another Point is, that the user can’t easily compare the Performance of the column. Overall ASTRA is equipped poorly with SEC Features. Another point is the creation of the molar differential distribution: with a linear calibration curve, there should be a same curve trend as in the RI-curve, e.g. a RI bimodal curve should create a bimodal diff. distribution. The diff. distribution doesn’t fit to the sample and isn't useful. My conclusion is the software should be more user-friendly. The after sales care is better, but most of the times it doesn't happen something after a phone call. It's stressful to call the service again, remind him and describe the problem once again. The value for money is ok. I mean there is an additional benefit. The hardware isn't bad. It's a great technology, but many bugs in the software reduce my satisfaction with the detector system. "

Review date: 24 Jun 2016 | DAWN®

Manufacturer's Response

Dear Dr.Engelkamp, Thank you for your valuable feedback about Wyatt’s instruments and your constructive critique of our ASTRA software. We trust that the majority of light scattering analyses fulfill your expectations for facile, robust and accurate determination of the absolute molar mass and size of macromolecules via SEC-MALS. We plan to address in future versions, those aspects of the software you perceive as unsatisfactory, while we continue to improve the user experience and add features across the product. We will be in contact with you to understand how the software could better meet your needs. Sincerely, Wyatt Technology Corp.
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Ultra Traces Anlyses Aquitaine - UT2A



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Very efficient tool for the characterization of nanomaterials, essential for our laboratory,
Rating: 4.7

  • Application Area:physicochemical characterization of nanomaterials

"The Wyatt Dawn Heleos II is a very effective tools for the characterization of nanomaterials (size; size distribution and molecular weight). Its implementation on an on-line analytical approach (ICPMS) is a strong added value for our technical center and services we propose. Most, the strong relationship between our company and the after sales and technical services from Wyatt Technology represents a high benefit to develop new analytical approaches."

Review date: 24 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Institute of Molecular Biology & Biotechnology (I.M.B.B) - Foundation of Resurch and Technology (FOR



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
It just makes my laboratory life much easier and much more effective.
Rating: 4.3

  • Application Area:Protein, Protein Complexes, Liposomes

"It's value for money since being so easy to use, I do use it almost every day having high quality reproducible results. "

Review date: 24 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Janssen Vaccines & Prevention B.V.



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Best Customer care ever!
Rating: 5.0

  • Application Area:Vaccine development

"All the instruments we use from Wyatt do exactly what they need to do and are very easy to use. Because Wyatt takes care of the complete package (HPLC; UPLC; MALS; QELS, RI) the customer care is exceptional!"

Review date: 24 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: r&D



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
very good material
Rating: 4.0

  • Application Area:food polymer (carrageenan)

"good equipment providing lot of info in an easy way"

Review date: 24 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Currenta GmbH & Co. OHG, CUR-ANT-MA



  • Ease of use
    3 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Very good Hardware, poor Software
Rating: 3.7

  • Application Area:Polymer Analytics

"The System itself has a very good quality, the signals and the reproducibility are very good. But the Software is only good for MALLS analytics, if you want to make other analytics like a universal calibration you will lost your nerves a time. We have an old System (Wyatt Dawn EOS) with the Software WinGPC Unity witch is much easier to use if you want to make a combined measurement of MALLS, Visko and Standard GPC. "

Review date: 24 Jun 2016 | DAWN®

Manufacturer's Response

Dear Dr.Szczesniak, Thank you for your valuable feedback about Wyatt’s instruments and your constructive critique of our ASTRA software. We trust that the majority of light scattering analyses fulfill your expectations for facile, robust and accurate determination of the absolute molar mass and size of macromolecules via SEC-MALS. We plan to address in future versions, those aspects of the software you perceive as unsatisfactory, while we continue to improve the user experience and add features across the product. We will be in contact with you to understand how the software could better meet your needs. Sincerely, Wyatt Technology Corp.
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Council fro Scientifc and Industrial Research



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Definitely cannot do without this instrument - it is the backbone of our work.
Rating: 4.7

  • Application Area:ANALYSIS OF CELLULOSE AND LIGNIN POLYMERS

"Great instrument that offers quality results. A change in software manipulations of the data that is different from the previous version has caused problems that are unique to our particular case. We are working with the manufacturer to get this resolved."

Review date: 24 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Janssen



  • Ease of use
    3 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Long-time user and fan of Wyatt products
Rating: 4.0

  • Application Area:vaccine and protein formulation development

"Great products, requires proper training and experience"

Review date: 24 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: praveen.agarwal@gennova.co.in



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Excellent
Rating: 4.7

  • Application Area:protein characterization

"Very good technology and excellent execution in terms of instrument. We were able to solve couple of very difficult problem with this technology, which otherwise was not possible. Very easy to understand, execute and interpret. "

Review date: 24 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: lupin limited



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great sturdy instrument with excellent after sales service!
Rating: 5.0

  • Application Area:Biosimilar protein characterization

"Dawn Helios II is an excellent product from Wyatt Corp. It’s very easy to use, understand the data and prepare report! We are using it for biosimilar protein characterization purpose. We have estimated the MW, Rh for multiple products very effectively using this machine. A great for every lab working on the protein characterization. "

Review date: 24 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Hannam University, Korea



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
I have used this instrument since 1990, and I cannot live without it.
Rating: 5.0

  • Application Area:Analyze nano-to micro-particles, macromolecules including bio and synthetic polymers, and dye, etc .

"I have used this instrument for long time since 1990. I have introduced this instrument to the 3M company, Minnesota in 1990, when I first joined the company. Now I am teaching in a college, and have one in my research laboratory. I am planning to purchase another one as soon as my budget allows. This instrument has been valuable tool for my work at 3M company, and also for my research in college. I am using this instrument as an either on- or offline detector for separation techniques such as field-flow fractionation and chromatography. It can provide important information on macromolecules including the size, molecular weight, molecular density, conformation, and so on. It helped tremendously for me to publish over 100 research papers in various international journals. This instrument is durable and easy to use (it was not that easy in its early development stage^^) "

Review date: 24 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Jiangsu University, Zhenjiang, Jiangsu, China



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Great results
Rating: 4.7

  • Application Area:Analyze the molecular weight and chain conformation of polysaccharides

"It is easy to use for us. We have successfully published some papers"

Review date: 24 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Univ. of Washington



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great instrument for polymer and protein analysis
Rating: 4.7

  • Application Area:Polymer and protein analysis

"Results are reproducible and the software is easy to use. Amazing customer service and quick response on application development questions."

Review date: 24 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Hawaii



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Overall it works fine but just not for the application I had in mind.
Rating: 4.0

  • Application Area:protein aggregation

"I do not use a flow cell but rather just a fixed volume. Although the system works well using the scintillation vial I have not had much luck with the low volume cuvette. That is just fuss intensive. "

Review date: 24 Jun 2016 | DAWN®

Manufacturer's Response

Dear Dr. Jameson, Thank you for your favorable review! The vast majority of DAWN HELEOS setups utilize the flow cell in on-line SEC-MALS or FFF-MALS flow mode, CG-MALS stop-flow injection mode or unfractionated flow injection mode. The DAWN’s flow cell provides an ideal optical environment for the most sensitive and robust measurements. However, if your sample is very viscous or sticky, has a tendency to gel or exhibit some other undesirable behavior, and you do not need fractionation for true distribution measurements of molar mass and size, then you are quite right to prefer a disposable scintillation vial and avoid the mess and labor of flow cell cleaning. The low volume quartz cuvette has much better optical quality than the scintillation vial and offers higher sensitivity with 500x lower sample volume, but as you noted it does require ‘fuss’ in the form of cleaning (since you are not going to throw away such a costly accessory). We’re glad we were able to offer a variety of options for the DAWN, one of which met your needs. Sincerely, Wyatt Technology Corp.
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: GlaxoSmithKline



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Thanks for the great customer service and timely responses to all of our questions!
Rating: 4.0

  • Application Area:Protein analysis, conjugation analysis, polydispersity analysis

"Working with the DAWN HELEOS II along with the T-REX RI detector has greatly improved our understanding of complex protein conjugates. We have also used the system to analyze polydispersity of our samples that are being purified for X-ray crystallography trials. This new instrument is a great asset to your group."

Review date: 24 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: UBC



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
The instrument has become integral part of our lignin research!
Rating: 5.0

  • Application Area:Lignin polymers

"We have achieved higher standard in characterization of lignin polymers using the SEC-MALS instrument. The outstanding service and technical support from Wyatt was one of the critical factor in that."

Review date: 24 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Fina



  • Ease of use
    2 out of 5
    After sales service
    5 out of 5
    Value for money
    3 out of 5
The after sales care is some of the best I've ever had.
Rating: 3.3

  • Application Area:Getting the molecular weight.

"Easy to use once you acquire enough training. I'm unfamiliar with the value for money statistic because I did not purchase it. The after sales care is some of the best I've ever had."

Review date: 24 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Eastman Chemical Company



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
A great instrument for providing accurate reliable data for the laboratory.
Rating: 5.0

  • Application Area:Analyze polymeric materials

"The instrument is very easy to use and adaptable to a multitude of solvents and polymeric materials. The service of the instrument is top quality in both the instrumental advice and consultation about methodology. A great staff of knowledgeable people with a true desire to assist with guidance."

Review date: 24 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: SDSU



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Producing results with ease
Rating: 4.7

  • Application Area:Dimerization

"I enjoy using the Dawn TREOS for my molecule weight analysis. I look forward to using the Nanostar DLS to quantify the binding interactions of the complex. Overall, I am looking forward to publishing my results and using the technologies of Wyatt. "

Review date: 24 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: The University of Akron



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great results, cannot live without this instrument!
Rating: 5.0

  • Application Area:polymer characterization

"All great - easy to use and gives great results. Wyatt people are very helpful."

Review date: 24 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Takeda San Diego (TCAL)



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Excellent product shaping the way to Great Innovational Findings
Rating: 4.3

  • Application Area:Determination of MW for Antibodies

"High quality, reproducible results, Easy to use Astra software, Great customer service and support,"

Review date: 24 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: UCLA



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Easy to use and obtain great, specific results!
Rating: 5.0

  • Application Area:Protein and nanoparticle characterization

"The results from this instrument are reproducible, specific, and it is incredibly easy to use and maintain."

Review date: 23 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2014

  • Organization: Siemens



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
A great product to own and a great company to work with.
Rating: 5.0

  • Application Area:Polymers& proteins

"The Heleos II MALS was well designed and robust. Customer service representatives are courteous and knowledgeable."

Review date: 23 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of New Mexico



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great results, can't live without this instrument!
Rating: 5.0

  • Application Area:Alayze metabolites in tissue samples

"Great product. Very good value for money. Wyatt Technology is consistently offering high quality products."

Review date: 23 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Michigan State University



  • Ease of use
    3 out of 5
    After sales service
    4 out of 5
    Value for money
    3 out of 5
This is a nice supplement to our standard approaches for protein characterization.
Rating: 3.3

  • Application Area:native molecular weight of proteins

"The instrument does much more than we need for nearly all of our applications. It was daunting to create a protocol for our needs, but once we established a simplified method that matches our simple requirements it has been easy to show others how to use it in the same manner."

Review date: 23 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: TU Dortmund



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Great results and great reproducibility, would not want to live without the equipment
Rating: 4.7

  • Application Area:Analyze bimolecular interactions, determining osmotic coefficients

"Although in the upper price range of light scattering equipment on the market the equipment is more than worth the money. Quality of the equipment, reproducibility of results and effectiveness are great. The support is above average."

Review date: 23 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Valspar



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
This instrument is invaluable in our lab. Wyatt is a great company and helps to make sure we have access to all the knowledge they posses.
Rating: 4.7

  • Application Area:Characterization of Polymers for Paint and Coatings Applications

"The MALS along with the Viscostar II and T-rex have given our lab a powerful tool in characterization of unknown polymers. We have been successfully characterizing raw materials, unknowns, and problem batches for our company for over a year now. We now can also get accurate polymer MW and distribution data where before we could only compare to standards (usually a dissimilar polystyrene). The system does require a relatively skilled person to perform analyses, but Wyatt also paid for training! Every time I have needed any help the highly trained Wyatt staff have answered questions very quickly. The support is wonderful."

Review date: 23 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: McGill



  • Ease of use
    3 out of 5
    After sales service
    3 out of 5
    Value for money
    3 out of 5
Good results but not always optimal.
Rating: 3.0

  • Application Area:Analyze of proteins aggregation or multimer

"A lot of noise in the signal and it is not easy to have a full window of the recording on the instrument itself. It is possible with the software but it will be useful to have it from the window of the instrument. I don't like the way to select the different options in the instrument."

Review date: 23 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: CNRS



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    3 out of 5
We are very pleased by this instrument !
Rating: 4.0

  • Application Area:Characterization of polymers

"This detector is very effective for the analysis of water-soluble polymers with very high molecular weights (and very low also). The results (Mn, Mw, D, Rg) are reproducible and of high quality. When there is a problem, it comes from very rarely from this detector but from SEC columns, pumps...!"

Review date: 23 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Ottawa



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
This is a necessary tool in our laboratory that we depend on. We feel confident in the results we get.
Rating: 4.3

  • Application Area:Analyze acrylic polymers for paints, coatings and adhesives.

"We find the equipment to be highly reliable. We have had many issues with the DRI (T-Rex) and the Viscometer requiring service but the MALS is working very well."

Review date: 23 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Pfizer



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Very useful detector in our analysis toolbox
Rating: 4.0

  • Application Area:Antibody Drug Conjugate analysis

"We use the instrument intermittently primarily because we are not able to control Astra via our enterprise Empower software. However, I would like to use it on a day-to-day basis and expand its use beyond ADC analysis i.e. nano-particle characterization"

Review date: 23 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2014

  • Organization: Solenis LLC



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Each SEC/GPC Lab has to have at least one set-up.
Rating: 5.0

  • Application Area:Polymer analysis

"Easy to use equipment. Always can rely on help from Manufacturer network. Reliable results and very good reproducibility. The set-up works 24/7/365."

Review date: 23 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Case Western Reserve University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
The workhorse of our laboratory
Rating: 5.0

  • Application Area:Polymer and Polymer-Grafted Nanoparticle Characterization

"The instrument is fantastic and sensitive. It gave us results that we just could not get any other way. We've since had outside users characterize some very exotic samples on the Heleos II and they are very impressed with the molecular weight and size characterizations. This is definitely the top of the line in light scattering detectors."

Review date: 23 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Dept of pharmacology, Unic. of Wash.



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Good robust instrument, and very reproducible
Rating: 5.0

  • Application Area:Absolute Mw of proteins

"Very easy to use. When used with size exclusion chromatography gives absolute Mw and purity of proteins. I use it to follow the effect of ligands on protein association. "

Review date: 23 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Prolong Pharmaceuticals



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Excellent for analysis of ADC and other Protein conjugates.
Rating: 4.7

  • Application Area:Pegylated protein analysis

"We use it for PEGylated protein analysis. It is a complete system with Agilent SEC and Optilab T-rEX. There is a steep learning curve for understanding the principles and mastering the data processing, however for us this has been one of the most reliable instrument providing accurate data. It has also been quite precise for method development. Extremely high sensitivity and complexity of the instrument make it highly vulnerable to slightest lack of care and maintenance. However, Wyatt service has been extremely helpful with providing useful information on use and maintenance of their products. We have also found the Wyatt online library to be of great help for understanding the applicability of the instrument for applications beyond what we are using it for."

Review date: 23 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Univ de Montréal



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
We would be hard-pressed to do without this instrument.
Rating: 5.0

  • Application Area:Protein characterization

"We chose the Wyatt MALS/RI instrument for three reasons: the ability to determine the absolute molecular weight of macromolecule, to assess monodispersity or conformational heterogeneity, and to be able to estimate the composition of protein conjugates. This has allowed us to routinely characterize the composition of protein detergent complexes which would otherwise been difficult to assess otherwise. The measurement protocols are easy to follow and greatly simplify protein characterization. Results are presented in an informative manner. "

Review date: 23 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: King Abdullah University of Science and Technology



  • Ease of use
    4 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
Quite reliable instrument if well maintained. Wyatt should extend their sources and branch out in the middle east and promptly inform customers in that region of any partnership with local vendors
Rating: 3.7

  • Application Area:Synthetic polymers, proteins

"The system is easy to use and provide reliable results. The challenge with after-sales service is the lack of competent service engineers in the Kingdom of Saudi Arabia for maintenance and service programs. Wyatt should engage a local vendor with trained engineers to assist customers in both preventative and corrective maintenance. Supply of parts and consumables for normal optimum running of equipment should also be contracted to local vendors."

Review date: 23 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: BMS



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Great instrument, really simplifies my life.
Rating: 4.3

  • Application Area:used for SEC-MALS to understand protein aggregation

"The product itself is quite robust and easy to use. The software is a little cumbersome and could be more intuitive. Otherwise, a great product."

Review date: 23 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2008

  • Organization: pfizer



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
We can’t live without it, so we keep 2 spares, so that at least one is always up.
Rating: 4.0

  • Application Area:glyco-conjugates

"SEC-MALLS is a powerful technique that is indispensable for certain applications, monitoring glyco-conjugates being one of those applications. Without SECMALLS we would be still be doing gravity columns and colorimetric analysis of fractions. I just wish we had less repairs with these instruments, we have averaged 2-3 weeks of instrument downtime a year. (n =9, 6 years)"

Review date: 23 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Montreal



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Highly recommended!
Rating: 4.7

  • Application Area:Environmental nanomaterials

"This is an excellent instrument, but without a doubt one of the best companies that I have worked with in over 30 years. They care not only about the sale, but they want to be sure that the client gets good results. Highly recommended!"

Review date: 23 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Alberta



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Best instrument with FFF
Rating: 4.3

  • Application Area:Size based separation

"Easy to use instrument and software, Good technical support, but difficult to repair at work place "

Review date: 23 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: MedImmune



  • Ease of use
    3 out of 5
    After sales service
    5 out of 5
    Value for money
    3 out of 5
Great instrument and fantastic customer care
Rating: 3.7

  • Application Area:Biophysical characterization of recombinant proteins

"I'm very happy with this instrument. We obtain high-quality results and the interface, analysis software and instrument capabilities are significantly better than many other light scattering instruments I have used. Also, the quality of technical assistance provided by Wyatt staff is absolutely amazing. Probably the best customer service I have ever received from an equipment manufacturer."

Review date: 23 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: FUJIFILM Diosynth Biotechnologies U.S.A., Inc.



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
This instrument is invaluable to the work we do for our clients and it makes science fun!
Rating: 5.0

  • Application Area:Analysis of protein biotherapeutics; batch MALS, SEC-MALS and CG-MALS

"The equipment produces high quality data that is reproducible and invaluable to our development of biologics. The training on how to run the instrumentation is top quality provided by scientists that truly care that you understand the related applications performed by this instrument. The staff at Wyatt are always eager to help in any way that they can whenever I have a question about maintenance, data analysis and troubleshooting. Some of the applications that can be performed with the MALS instrument can be complex. It is wonderful to know that there is always someone there to help me understand both the science and the how-to's of running an experiment."

Review date: 23 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: bioMérieux



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Best dRI detector of the world !
Rating: 4.7

  • Application Area:Biotechnology, raw material characterization

"Coupled with a MALS and UV-visible detectors, the Optilab T-rEX push the limits of the biomolecules detection and quantification. Both ultrasensitive and ultra-broad dynamic range, no matter the sample concentration. There anyway a little flipside to that coin, to get a perfect baseline you need a perfect regulation of your LC pump, sometimes out of the range of the LC manufacturers AQ specs. But this is not a big deal, some optimizations of the LC system have just had to be done. "

Review date: 23 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: MilliporeSigma



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
We get great results. Wish we had two of them
Rating: 5.0

  • Application Area:Used for aggregate analysis for both upstream and downstream. To look at causes and removal.

"We are very happy with this product. The results are very reproducible."

Review date: 23 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Bristol-Myers Squibb



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
great results and great technical support
Rating: 4.3

  • Application Area:antibody, antigen, ADCs size analysis

"The system is easy to use and I achieve high quality reproducible results. Every time I have a problem related to the software or the instrument, technical support is able to answer my questions very efficiently. "

Review date: 23 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Chevron Oronite



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Depended on the quality of the instrument and customer service, looking forward for lower prices and enhance operating software
Rating: 4.3

  • Application Area:Analyze polymers

"Excellent reproducibility, versatile system, but the software (methods, running, analyzing data) can be improved toward "user friendliness""

Review date: 23 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Radiation Physics Division, NIST



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Great machine! It does the job, as intended...
Rating: 4.3

  • Application Area:Extract nanoparticle dimensions for suspensions

"This product has served its purpose well over the last several years. Multiple users from various parts of NIST have used it successfully for their projects. This was the intended purpose for the purchase."

Review date: 23 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: bioMérieux



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Dawn Heleos 8+ fits perfectly for our needs
Rating: 4.7

  • Application Area:biotechnology / raw material characterization (proteins, polysaccharrides, particles, VLP)

"Brand new equipment in our lab (installed October 2015) in use with HPLC pump + Eclipse Dualtec and coupled with UV-visible and dRI (Optilab T-rEX) detectors, our Dawn Heleos 8+ MALS detector allow us to perform fine characterization of biomolecules we never could study before and allows us to support the development of our new immunoassay reagents that make us to head increasingly to particles and polysaccharides as antigens. Its use is very simple thanks to fast start-up of the instrument and the perfect Astra software integration. The built-in Comet ultrasound sonicator enable a perfect cleaning of the cell. Everything is perfectly well designed and focused to make life easier for the user. I would also mention the rapid and efficient support from the application specialists team. Very great instrument!! "

Review date: 23 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Cu Boulder



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
A very good instrument - I wish they would support it longer than 8 years.
Rating: 4.3

  • Application Area:Analyze proteins, protein complexes and Protein DNA complexes

"We get very good results and very reproducible results with our instrument. It is easy to use in principle. But in a multi user environment it is hard to keep the MALS detector scrupulously clean which is a prerequisite for good data. That has absolutely nothing to do with Wyatt or the quality of the instrument but is a fact of life for Light Scattering experiments. So it is not an instrument that everybody can be easily trained on just because the measure on carful sample and solvent preparation and on carful treatment of the SEC system connected to the MALS this is hard to drum into people. Consequently, because we have multiple users we have quite often issues with cleaning detectors but the customer service from Wyatt is incredible and has so far been amazing helping me to sort the stupid things out that happen with multiple users. Especially if used exclusively by a well-trained, meticulous person I don't think the Wyatt MALS systems can be beaten. And The LS University goes a long way to insure training and understanding of instrument, method and limitations. I only wish the support would be longer than 8 years. In an academic setting it is really next to impossible to raise money to update instruments with this frequency! That is my only negative comment for support. "

Review date: 23 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: York University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
The system is a great resource that provides excellent analytical data.
Rating: 5.0

  • Application Area:Protein (monomer and oligomeric) analysis in solution

"We've had the HELEOS and Optilab TrEX for several years, and have found the systems to be very robust and provide excellent data."

Review date: 23 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Celgene



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Best on the market!
Rating: 5.0

  • Application Area:Characterization of biotherapeutics

"Our DAWN Heleos II is by far the best SLS instrument on the market. The Astra software is very intuitive and user-friendly. The best part of the instrument is the support I have received from the entire Wyatt company."

Review date: 23 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Takeda Vaccines, Inc.



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Solid results, great support.
Rating: 4.7

  • Application Area:Protein characterization, vaccines

"This instrument and technology has been critical in helping characterize materials used in our vaccine program. SEC-MALS has provided some novel insight into the composition/nature of one of our products which would not have been accessible with alternative techniques (and definitely not with SEC alone). We are in the process of relocating and expanding our operations; this instrument is at the top of the list to duplicate so we can continue to achieve our goal of robust characterization of molecules in our pipeline."

Review date: 23 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: NNF CPR



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
The results are robust and reproducible.
Rating: 5.0

  • Application Area:molecular size of proteins, protein/DNA complexes, protein/carbohydrate conjugates

"SEC-MALS is the technique of choice in our lab to characterize our proteins and complexes in terms of their molar mass. We use either the Opti-rEX and UV or both detectors as a source for the concentration depending on our applications. The instrument is easy to use and taking the precaution of preparing fresh solutions and filtering running buffers and samples, the instrument runs smoothly most of the time. "

Review date: 23 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: CIAD A. C.



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Excelent equipment
Rating: 4.0

  • Application Area:Biopolymers (polysaccharides, proteins) characterization

"All the Wyatt detectors are fine pieces of equipment, functional and reliable. They have increased our analytical capacity "

Review date: 23 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: BASF CC ITALY



  • Ease of use
    4 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
Great results, powerful instrument.
Rating: 3.7

  • Application Area:Polymer analysis

"Polymer analysis and characterization; reliable MW results. Instruments: easy to use. Astra Software: not user-friendly as expected, anyway it is a very powerful system/tool. LS calibration: easy and reliable as well."

Review date: 23 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Lund University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
I had good results
Rating: 5.0

  • Application Area:macromolecules

"Asymmetrical flow field-flow fractionation when connected to suitable detector such as multi-angle light scattering (MALS) has shown to be a great technique for the analysis mAsymmetrical flow field-flow fractionation when connected to suitable detector such as multi-angle light scattering (MALS) has shown to be a great technique for the analysis molar mass and root mean square of many food macromolecules. The results are reproducible and with good quality olar mass and root mean square of many food macromolecules. The results are reproducible and with good quality"

Review date: 23 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: MilliporeSigma



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
The best instrument in the analytical chemistry business with even better customer support.
Rating: 5.0

  • Application Area:Analyze antibody-drug conjugates and other bio-conjugates for MW and polydispersity.

"The DAWN HELEOS II MALS detector is one of the best instruments I have ever used in my many years in analytical chemistry labs. A very easy to use instrument that gives reliable results. I can depend on this instrument day in and day out. Not only is the instrument outstanding, but the customer service is bar none the best I have ever come across. No matter what question or issue I have, Wyatt's customer service has an answer that works. I will be using this instrument and working with Wyatt for the rest of my career. All other companies have a lot to improve on to get even remotely close to the level Wyatt is at. Thanks for your amazing products and your even better customer service."

Review date: 23 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Laurentian University, Canada



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    5 out of 5
Indispensable to my research lab. A major work horse in the lab
Rating: 4.3

  • Application Area:Polymer samples

"The instrument has been highly reliable after 9 years of heavy use, with no services required from the company. With the instrument, we have published many papers in good journals of my field."

Review date: 23 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2014

  • Organization: Teva



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Great instrument easy to work with but sensitivity should be improved
Rating: 4.7

  • Application Area:Peptide aggregate characterization

"I have used this detector for polymer analysis and peptide aggregate analysis. other than for small peptides I liked the unit. for smaller peptides with low concentrations I have very hard time to get the sensitivity. "

Review date: 23 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Kansas



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Very user friendly. On top an excellent Tech support.
Rating: 5.0

  • Application Area:Protein and macromolecules stability

"This instrument is very helpful to measure absolute molecular weight of products which is unaffected by relative separation of the components. Also, we use it for routine protein and macro-molecules stability analysis. It’s an excellent and easy to use instrument. "

Review date: 23 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Montreal



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
good instrument
Rating: 4.3

  • Application Area:nanoparticles characterization

"Easy to use in batch-line with a syringe pump. Calibration is a little bite challenging at the beginning but when you used to it is ok. Coupling Optilab and Heleos give interesting information on your samples"

Review date: 23 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Allergan



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Good as we expected
Rating: 5.0

  • Application Area:Measure MW of polymers

"The results met our expectation. The system is reliable. We used the data generated to make critical decisions."

Review date: 23 Jun 2016 | DAWN®
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Pfizer



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Must have!
Rating: 5.0

  • Application Area:Protein and Polysaccharide

"Easy to use and maintain instrument. Great training program that helps with figuring out all the ways it can be utilized"

Review date: 20 Jun 2016 | DAWN®

The DAWN® multi-angle light scattering (MALS) detector connects to most HPLC, GPC, FPLC or FFF separation systems to determine absolute molecular weight, size, and conformation of peptides, proteins, polymers and nanoparticles, in solution, without reference to calibration standards. 

Eliminate column calibration and idealized assumptions, gain certainty with first-principles MALS analysis. With 18 detection angles, superb optical engineering and a range of optional add-ons, the DAWN provides the highest sensitivity, range of measurement and versatility of any available MALS detector. 

Features: 

  • Sensitivity in SEC-MALS mode: 10 ng of 100 kDa polystyrene in THF, or 200 ng of BSA in PBS, using 7.8 x 300 mm SEC columns 
  • Range of molar mass: 200 Da to 1 GDa (may vary with molecular composition or solvent) 
  • Range of rms radius Rg : 10 nm to 500 nm (up to 1000 nm with specific shape models) 
  • Temperature-controlled models: ambient; H/C, -15°C to +150°C; UHT, room temperature to +210°C 
  • Integrated ultrasonic cell cleaning module
  • DLS: optional WyattQELS™ embedded dynamic light scattering detector for particle sizes from 0.3 nm to 300 nm (Rh
  • Optional fluorescent-molecule configuration includes infrared laser and fluorescence-blocking filters
  • Touch-panel display for immediate access to data, diagnostics and settings
  • Serviceability: fully field-serviceable with extended self-diagnosis of field-replaceable flow cell and hardware modules
  • Software: ASTRA® chromatography software provides comprehensive analyses and user-friendly features including HPLC control, “One-click MW” and user-designed reports. Optional 21CFR11 support for GMP.

Applications:

  • Proteins: absolute molar mass, size and stoichiometry of monomers, oligomers, complexes, aggregates and fragments.
  • Glycoproteins, detergent-solubilized membrane proteins, PEGylated proteins, protein-polysaccharide and protein-DNA complexes; heme-containing proteins that absorb laser light
  • Protein-protein and protein-nucleic acid interactions
  • Polymers: absolute molar mass and size (distributions and moments), conformation, branching and second virial coefficient
  • Linear or branched polymers, co-polymers, fluorescent polymers such as lignin
  • Nanoparticles: size distributions, structure, encapsulation and concentration of vesicles, liposomes, dendrimers, viruses, VLPs, fibrils and more
  • Most aqueous and organic solvents