Comprehensive chromatographic assessment of forced degraded in vitro transcribed mRNA

Heightened interest in mRNA therapeutics has accelerated the need for analytical methodologies that facilitate the production of supplies for clinical trials. This webinar will present a concise panel of chromatography-based stability-indicating assays for evaluating thermally stressed in vitro transcribed mRNA as part of a forced degradation study.

Our expert speakers will demonstrate the application of these methods to evaluate the critical quality attributes (CQAs) of mRNAs as well as to detect intrinsic process- and product-related impurities.

Key learning objectives

• Characterization of thermally stressed mRNA using a panel of chromatographic assays
• Implementation of SEC-MALS to confirm transcripts reflect full-length sequence design
• Platform 5’ cap/3’ tail analyses, circumventing antisense probe design
• Design of QC-friendly methods fit for research, release, and comparability testing

Who should attend?

• QC lab technicians or lab assistants.
• Lab managers of development, process development, or QC.
• Directors/Senior directors of development, process development or QC
• VP of Quality; VP of Development or VP of CMC.
• Anyone interested in learning more about light scattering and its applications on mRNA characterization.
• Industry and academic researchers working in gene therapy and biotherapeutics.
• Any scientist in discovery, development, and quality control.

Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.