Biomek NGeniuS Next Generation Library Prep System

Next-generation sequencing (NGS) is a high-throughput DNA sequencing technology that enables rapid and cost-effective analysis of large amounts of genetic information. Unlike traditional Sanger sequencing, NGS allows for the simultaneous sequencing of millions of DNA fragments, providing a more comprehensive and detailed view of an organism’s genetic makeup.

Despite its many advantages, NGS workflows also face several challenges, which can affect the quality and reliability of the generated data. Automated sequencing preparation can help solve several challenges in the NGS workflow by streamlining and standardizing the sample preparation process, reducing manual processing errors, and increasing efficiency. This eBook provides an informative guide on how automated solutions can be leveraged to optimize your NGS workflows for the following fields:

• Whole genome sequencing
• Metagenomics
• Transcriptomics
• Targeted capture and enrichment
• Exome sequencing
• Single-cell sequencing

In the past three decades, methods for sequencing nucleic acids have improved significantly in speed and accuracy. Despite the technological advances in sequencing, preparation of libraries for Next-generation sequencing (NGS) remains a tedious process. In this application note, Beckman Coulter Life Sciences demonstrates that the automated processing on the Biomek NGeniuS Next Generation Library Prep System is equivalent to the manual preparations at input concentration of 200 ng (gDNA) and 200 ng (FFPE DNA). The hands-on time for processing is reduced, handling errors are reduced, and the interactions/touchpoints are reduced.

Automation reduces the errors associated with next generation sequencing (NGS) sample preparation by decreasing sample-to-sample variation, but not all automated liquid handlers have the same hardware and software capabilities to reduce human errors. This application note compares a Beckman Coulter Next Generation Library Prep System and a traditional low-throughput liquid handler.

The benefits of switching from manual to automated NGS library preparation are many. Automated NGS is more efficient, less prone to human errors (such as during pipetting), less susceptible to contamination, and more reliable and consistent. However, not all liquid handlers are equipped with similar hardware and software features to provide all the benefits of automation. In this application note, a traditional liquid handler was compared with the Beckman Coulter Biomek NGeniuS Next Generation Library Prep System.

This application note details an automated process for the IDT xGen CcfDNA & FFPE DNA Library Prep MC kit that offers the laboratory optional settings to optimize a demonstrated application that will process between 4 and 24 samples from start to finish with minimal interaction from the user. Library preparation results using the IDT xGen cfDNA & FFPE DNA Library Prep MC kit on the Biomek NGeniuS Library Prep System are described.

Laboratories are looking for reproducible next generation sequencing (NGS) sample preparation methods that limit the potential for in-process sample degradation or error. This application note details an automated process for the New England Biolabs NEBNext Ultra II Directional RNA Library Preparation Kit for Illumina that offers the laboratory optional settings to optimize a demonstrated application that will process between 4 and 24 samples from start to finish.

Next-Generation Sequencing (NGS) is rising in popularity as the costs of applying this technology to genome sequencing fall. Laboratories are looking for highly reproducible NGS sample prep methods that limit potential for error. In this application note, Beckman Coulter Life Sciences details an automated process for the Illumina DNA Prep library preparation kit that offers the laboratory optional settings to optimize a demonstrated application that will process between 4 and 24 samples from start to finish with minimal interaction from the user.

Preparing next-generation sequencing (NGS) libraries manually can be labor-intensive and time-consuming, taking anywhere from a few hours to several days depending on the type of library being created.

Join Dr. Jeffrey Wilson and Zach Smith, Staff Application Scientists at Beckman Coulter Life Sciences, as they demonstrate the automation of two distinct NGS library preparation kits using the Biomek NGeniuS System utilized in oncology research.

The Pillar Biosciences oncoReveal Solid Tumor 22 Gene Panel Kit utilizes proprietary amplification technology and a small panel targeting 22 genes to amplify the target regions. The Illumina TruSight Oncology 500 DNA/RNA kit uses an alternative strategy involving NGS library preparation followed by hybridization/capture using a much larger panel of 523 genes along with other markers to develop a comprehensive genomic profile of the sample.

Key learning objectives:

• Gain insights into the features and automation capabilities of the Biomek NGeniuS system, including its role in laboratory efficiency
• Explore the Pillar and TSO 500 library preparation workflows, including the specific cancer variants and mutants they target for enrichment and amplification
• Learn how the Biomek NGeniuS system can manage two distinct workflows, highlighting its flexibility and adaptability in different research settings
• Examine the data generated from automating the Pillar and TSO workflows on the Biomek NGeniuS, and the implications for cancer testing laboratories, including improvements in accuracy and efficiency

Who should attend?

• Professionals involved in cancer research, including researcher, academics and clinical researchers.
• Those interested in NGS and automation.

Certificate of attendance

All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.

Assay automation from Beckman Coulter Life Sciences and Illumina makes cutting-edge research more accessible to labs

Learn how to free up your researchers and minimize errors with flexible, walk-away automated NGS library preparation

The new state-of-the-art complex further strengthens its commitment to the greater Loveland, Colorado community

Next generation sequencing (NGS) solutions will be highlighted