Automated NGS library preparation for oncology application using the Biomek NGeniuS System

Preparing next-generation sequencing (NGS) libraries manually can be labor-intensive and time-consuming, taking anywhere from a few hours to several days depending on the type of library being created.

Join Dr. Jeffrey Wilson and Zach Smith, Staff Application Scientists at Beckman Coulter Life Sciences, as they demonstrate the automation of two distinct NGS library preparation kits using the Biomek NGeniuS System utilized in oncology research.

The Pillar Biosciences oncoReveal Solid Tumor 22 Gene Panel Kit utilizes proprietary amplification technology and a small panel targeting 22 genes to amplify the target regions. The Illumina TruSight Oncology 500 DNA/RNA kit uses an alternative strategy involving NGS library preparation followed by hybridization/capture using a much larger panel of 523 genes along with other markers to develop a comprehensive genomic profile of the sample.

Key learning objectives:

• Gain insights into the features and automation capabilities of the Biomek NGeniuS system, including its role in laboratory efficiency
• Explore the Pillar and TSO 500 library preparation workflows, including the specific cancer variants and mutants they target for enrichment and amplification
• Learn how the Biomek NGeniuS system can manage two distinct workflows, highlighting its flexibility and adaptability in different research settings
• Examine the data generated from automating the Pillar and TSO workflows on the Biomek NGeniuS, and the implications for cancer testing laboratories, including improvements in accuracy and efficiency

Who should attend?

• Professionals involved in cancer research, including researcher, academics and clinical researchers.
• Those interested in NGS and automation.

Certificate of attendance

All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.