NGS for high quality and consistent ‘omics results: Your essential guide

3 Apr 2023

Next-generation sequencing (NGS) is a high-throughput DNA sequencing technology that enables rapid and cost-effective analysis of large amounts of genetic information. Unlike traditional Sanger sequencing, NGS allows for the simultaneous sequencing of millions of DNA fragments, providing a more comprehensive and detailed view of an organism’s genetic makeup.

Despite its many advantages, NGS workflows also face several challenges, which can affect the quality and reliability of the generated data. Automated sequencing preparation can help solve several challenges in the NGS workflow by streamlining and standardizing the sample preparation process, reducing manual processing errors, and increasing efficiency. This eBook provides an informative guide on how automated solutions can be leveraged to optimize your NGS workflows for the following fields:

  • Whole genome sequencing
  • Metagenomics
  • Transcriptomics
  • Targeted capture and enrichment
  • Exome sequencing
  • Single-cell sequencing

Echo® 525 Liquid Handler

Beckman Coulter Life Sciences

The Echo 525 platform, like the other Echo liquid handler models, is designed to use sound energy to transfer reagents. Unlike the other Echo models, the Echo 525 platform transfers at a larger volume increment (25 nL). This enables the more rapid transfer of aqueous reagents required in biochemical and genomics assays setup.

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Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Target-EnrichmentNGS SoftwareMetagenomicsTranscriptomicsNGS AutomationNGS automation streamlines next-generation sequencing workflows, increasing throughput, accuracy, and reproducibility. Automated solutions enhance productivity in genomic research, clinical diagnostics, and personalized medicine. Browse peer-reviewed tools to compare products, read reviews, and get pricing.Single Cell GenomicsGenome SequencingGenome sequencing involves determining the complete DNA sequence of an organism's genome. It provides crucial information about genetic variations, mutations, and diseases. Advances in sequencing technologies, such as next-generation sequencing (NGS), have accelerated research in genomics, diagnostics, and personalized medicine. Explore genome sequencing tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Exomes