DAWN® by Wyatt Technology Corp.

"We are very happy with the purchase of this Wyatt MALS detector. It is the workhorse for polymer characterization in our lab. Great tool and relatively easy to use."

"Great product and excellent customer service. The instrument is easy to use and integrates well with other products."

"DAWN is an easy to use multi angle light scattering detector. It allows you to analyze the molecular size with in-line with SEC and thus, we can visualize changes in size easily on protein-protein and protein ligand interactions."

"I have been using MALS since the 1980's - Wyatt's have always been the best value. This latest is very user-friendly. The combined information from the MALS and the viscometry detector can't be beaten. Branched co-polymers are no problem; great for pH."

"This is the only technology available for absolute molecular size determination with ease of use. It is highly reproducible and recommended for polysaccharide and conjugate vaccine manufacturers."

"Easy to use to determine protein oligomerization state with connection to FPLC it is possible to check fraction on SDS-PAGE gel."

"It is very effective in measurement of the molecular mass of a protein in-line to SEC. We are able to differentiate small changes in size. It is easy to use instrument."

"Use of MALS coupled with RI detector for the analysis of polysaccharide is outstanding if the separation technique is sufficient. Service after sales is simply the best. If you want to characterize large biomolecules, it is one of the best options."

"The instrument is very easy to use and the results are reproducible provided you take care to ensure that you have your sample in exactly the same buffer as that used for the MALS experiment. It enables a faster initial estimation of protein complex stoichiometries than is available by Analytical Ultracentrifugation and it is extremely useful for estimating the oligomeric state of membrane proteins by separating the protein and detergent components. We now routinely use the instrument to assess the oligomeric state and purity of all proteins we work with and we have incorporated it into all our protein purification SOPs."

"This detector couple with dRI detector are a very useful tool for characterizations of macro molecules and particles as well."

"DAWN HELEOS II is one of the best equipment for size , molecular mass and number density characterization. Along with SEC/FFF this proves to be a highly advanced technology for comparability and analytical characterization of different biological molecules."

"Very easy to use. Using only THF, the instrument is very reproducible and is requires minimal upkeep. Results match other techniques reasonably."

"We have been very pleased with the instruments performance and ease of use. It has given us valuable information on the oligomeric states of the proteins and protein nucleic acid complexes that we are trying to crystallize."

"The 18 angle LS detector works really well and I am overall pleased with its functionality. However, for this application 18 angles is definitely overkill. It slows down our GPCone software and leaves more opportunities for malfunctions. So far we have not had to make any repairs, but I have heard that making repairs on this system can be very difficult. It would be nice if they made a simpler, easy to repair, 3-6 angle detector for this type of application."

"Great instrument. Solid robust performance. Instrument is fairly easy to use after training and easy to implement into workflows."

"We have implemented this method as the quality control for our protein conjugates. the results are included in FDA approval submission. The conjugates products are used as vaccine in a clinical trial."

"The Wyatt staff has been extremely helpful in assisting us in our applications. "

"It is a fantastic MALS detector, easy to use and vary reliable. I have used/seen this detector in many labs, always happy to work with this MALS detector."

"The results that we achieve are very high quality and the instrument is easy to use."

"Great instrument. Much better than previous instrument that I've used."

"The MALS detector produced critical data that allowed for routine QC of the target protein for our drug discovery program"

"If you are still uncertain about the limitations of SEC, well it's time for you to learn about them. There is no protein biophysics without knowledge of molecular mass in solution. That is such a critical and central piece of information to have on your proteins and no instrument out there will give you a better estimate of it than the Heleos. Wyatt customer since 2008, couldn't be happier about the analytical power they provide."

"Without the DAWN HELEOS II, we'd be skeptical of our molecular weight measurements by SEC and column calibration alone. The DAWN allows us to rest assured that we are getting reliable data. Wyatt has also been exceptional with our unusual application requests."

"The instrument allows the researcher to determine the degree of PEGylation of antibody."

"Great instruments for the characterization of molecular structure of polymers. Especially useful for the identification and characterization of degree of branching. I appreciate the reproduciblity of obtained results."

"Once the product is set up and calibrated it is very easy to use, even with large amounts of time in between not being used. The instrument is very reliable and accurate. Once it has been calibrated my control standard is very reproducible. The phone help is excellent, there is no wait time and they will do everything necessary to answer your questions or fix your instrument. When I send my instrument in for yearly service it is completed in a reasonable time frame and any work done is fixed and report to me."

"This instrument is perfect for understanding protein/detergent complexes and ascertaining the stoichiometry of protein/protein complexes. Wyatt also offer an excellent training course which is backed up by an excellent support team who are always on hand to help out."

"The DAWN HELEOS is a technologically advanced and well-designed light scattering system. When a glycoprotein won't "fly" in mass spec, we use the HELEOS in triple detector mode to characterize the amount of glycosylation. As a first principle method returning mass, it is useful for any all proteins and tight complexes of macromolecules. The Wyatt flow cell and software are an excellent way to implement this method. "

"HELEOS is a highly professional and easy to use detector of our AF4 device. The possibility to use it together with a refractive index detector Optilab rEX in a batch/stand-alone mode is an excellent feature. It is really easy to convert HELEOS and Optilab rEX from detectors into high quality scientific devices. We work with thermally responsive polymers and HELEOS with precise temperature control is just great. Importantly, the results are obtained practically instantaneously, especially if compared with goniometer-type instruments. We also receive very quick and professional help from Wyatt services: we are specialists in light scattering but we ask for help in respect to usage of AF4 and software (Astra). For potential customers: LS is a highly informative but difficult method. Wyatt products can do much more than an average user can imagine. In my opinion, a physicist specialized in polymers/particles in solutions should run AF4, HELEOS and Optilab rEX. It is not enough to run an experiment. One should know physical properties of polymers in order to design the experiment correctly. Only then the true value of Wyatt instruments can be appreciated."

"The instrument is very useful on the application of pharmaceutical researches, especially large molecular biological products. It has high precision and sensitivity. Presently there is no substitutional production at China market. It has friendly media and is relatively easy to learn how to use. It will be better if the company provide a ready-to-use instruction and same examples of how to utilize the instrument. "

"Excellent contacts with application lab in Germany. The scientists are helpful and responsive and very knowledgeable. Instrument needs expert handling (as many other instruments, though)."

"Very easy to use especially go through the training class on site at Santa Barbara Great customer support both online and by phone, detailed instructions on all trouble shooting materials Provide great and precise molecular weight based on both size and shape Instrument is very durable and seldom need repair Major pieces of equipment for my project and for publication "

"All of our Wyatt instruments perform very well. I have used detectors from a number of manufactures, and Wyatt has consistently been of the highest quality. Not only that, but the Wyatt customer service is the best. I wouldn't purchase anything else."

"The product is effective and we were able to find the particle sizes for liposomes and micelles as needed. We also had a tech. support visitor, visit our campus and help us understand all the functions of the equipment. "

"Instrument can be used by many researchers without major problems. MALS and DLS theory and the opportunities of the instrument could be presented in a more convenient way. Also, the main issues like high baseline noise and contamination could be summarized in a separate application note/ white paper (instead of reading the whole manual). I would appreciate more tutorial videos instead of written documents. "

"We obtained good results for the analysis of soluble proteins. However, for the protein conjugate analysis, I find very little documentation about the practical aspect of that kind of analysis. "

"We appreciate working with the instrument and our student learn a lot on macromolecules with that. The after sales care has always been perfect. The only thing that we don't fully appreciate is the software Astra 5, it's not really easy for a new person to understand it quickly. "

"This is an excellent MALS/DLS instrument that we use regularly."

"We have worked with Wyatt Technology multi-angle laser light scattering (MALLS) equipment for over 15 years. We have used this exclusively for aqueous polymer and biological fluid (equine synovial fluid) characterization in static and GPC-MALLS modes. This has been used by a wide variety of people at various levels of technological acumen. The fundamentals of light scattering may be difficult to learn, but the use of the Wyatt equipment to get good data is relatively easy for a careful experimentalist. I have sent 4 students to the Wyatt Light Scattering University (LSU) and this has been extremely beneficial. The Wyatt technical support has always been outstanding, not only on equipment operation, but also on helping to optimize and understand difficult experiments. Frankly, I would never consider buying polymer molecular characterization equipment from any other company. "

"Our MALS system from WYATT is coupled with SEC. The obtained results are high quality and reproducible, but preparation of the machine for run takes a lot of consumables. By a lot I mean ~5 column volumes, to have stable readout for light scattering and refractive index detectors."

"The Heleos II detector is durable and easy to use if one has previous experience with light-scattering instruments. Equilibration of the instrument is fast and it is very easy to switch between solvents. The ability of the instrument to revert to stand-by (idle) mode is particularly useful for running a sequence overnight when no one is around to oversee the experimental run. Maintenance is also easy. "

"The instrumentation and software work very well for our process and finished product needs. Very little maintenance needed and only occasional calibration checks required. "

"I'm using such products since more than 25 years, the results are always very robust and reproducible, the software allows to get a lot of useful information on size and shape of the molecule."

"The instrument is very reliable and working very well. It is a critical instrument for absolute molecular weight determination of polymer. Software is user-friendly."

"The HELEOS provides us with good reproducible results, I think the main issue we have is with our lack of suitable SEC columns for our wide variety of synthetic polymers! But this is where the real utility has been shown for the instrument, as we regularly run batch mode Zimm plot experiments to counteract this."

"Highly sensitive detector with very good s/n ratio"

"Really good instrument, only limited by the quality of our HPLC. Works even for relatively small proteins. Mostly used for working out what is going on with complex samples. Used for identification of multimers."

"Excellent results, no matter what the shape of the protein we get a spot-on MW value. One of the most popular techniques in our facility. Researchers now trust the results so much that they can identify ligands bound to proteins that they were not expecting. The courses at Santa Barbara were excellent. Would recommend going as soon as you got the instrument. Because it is so heavily used I would appreciate some sort of maintenance programme just for the confidence that we could get it repaired if anything did go wrong. "

"Since it was the first time for me to do polymer characterization using the Wyatt Optilab (TreX), Dawn Heleos and Viscostar the three days training I attended at Santa-Babra helped a lot in understanding the use of the detectors and better processing of results using the Astra Software. On our CMC samples we currently running we able to get accurate, reproducible and reliable results. Although we still experiencing some difficulties with our Polyacrylamides samples the help we getting from the Wyatt support team have been very helpful and we confident that all our problems will be resolved soon."

"We use SEC MALS dRI that gives us excellent data set on sample quality and heterogeneity. The multi-angle light scattering detector allows us to detect larger aggregates and segregate them out of vaccine solution. Additionally, we obtain hydrodynamic radious calculation as well as molecular weight of a protein. "

"The technology in the instrument DAWN HELEOS II is properly chose. The signals are clear and the measurement is sensitive. In the other hand the software isn't customer friendly. First the Option for a properly SEC Quality Management doesn’t exist. The creation of a calibration is time/consuming. Another Point is, that the user can’t easily compare the Performance of the column. Overall ASTRA is equipped poorly with SEC Features. Another point is the creation of the molar differential distribution: with a linear calibration curve, there should be a same curve trend as in the RI-curve, e.g. a RI bimodal curve should create a bimodal diff. distribution. The diff. distribution doesn’t fit to the sample and isn't useful. My conclusion is the software should be more user-friendly. The after sales care is better, but most of the times it doesn't happen something after a phone call. It's stressful to call the service again, remind him and describe the problem once again. The value for money is ok. I mean there is an additional benefit. The hardware isn't bad. It's a great technology, but many bugs in the software reduce my satisfaction with the detector system. "

"The Wyatt Dawn Heleos II is a very effective tools for the characterization of nanomaterials (size; size distribution and molecular weight). Its implementation on an on-line analytical approach (ICPMS) is a strong added value for our technical center and services we propose. Most, the strong relationship between our company and the after sales and technical services from Wyatt Technology represents a high benefit to develop new analytical approaches."

"It's value for money since being so easy to use, I do use it almost every day having high quality reproducible results. "

"All the instruments we use from Wyatt do exactly what they need to do and are very easy to use. Because Wyatt takes care of the complete package (HPLC; UPLC; MALS; QELS, RI) the customer care is exceptional!"

"good equipment providing lot of info in an easy way"

"The System itself has a very good quality, the signals and the reproducibility are very good. But the Software is only good for MALLS analytics, if you want to make other analytics like a universal calibration you will lost your nerves a time. We have an old System (Wyatt Dawn EOS) with the Software WinGPC Unity witch is much easier to use if you want to make a combined measurement of MALLS, Visko and Standard GPC. "

"Great instrument that offers quality results. A change in software manipulations of the data that is different from the previous version has caused problems that are unique to our particular case. We are working with the manufacturer to get this resolved."

"Great products, requires proper training and experience"

"Very good technology and excellent execution in terms of instrument. We were able to solve couple of very difficult problem with this technology, which otherwise was not possible. Very easy to understand, execute and interpret. "

"Dawn Helios II is an excellent product from Wyatt Corp. It’s very easy to use, understand the data and prepare report! We are using it for biosimilar protein characterization purpose. We have estimated the MW, Rh for multiple products very effectively using this machine. A great for every lab working on the protein characterization. "

"I have used this instrument for long time since 1990. I have introduced this instrument to the 3M company, Minnesota in 1990, when I first joined the company. Now I am teaching in a college, and have one in my research laboratory. I am planning to purchase another one as soon as my budget allows. This instrument has been valuable tool for my work at 3M company, and also for my research in college. I am using this instrument as an either on- or offline detector for separation techniques such as field-flow fractionation and chromatography. It can provide important information on macromolecules including the size, molecular weight, molecular density, conformation, and so on. It helped tremendously for me to publish over 100 research papers in various international journals. This instrument is durable and easy to use (it was not that easy in its early development stage^^) "

"It is easy to use for us. We have successfully published some papers"

"Results are reproducible and the software is easy to use. Amazing customer service and quick response on application development questions."

"I do not use a flow cell but rather just a fixed volume. Although the system works well using the scintillation vial I have not had much luck with the low volume cuvette. That is just fuss intensive. "

"Working with the DAWN HELEOS II along with the T-REX RI detector has greatly improved our understanding of complex protein conjugates. We have also used the system to analyze polydispersity of our samples that are being purified for X-ray crystallography trials. This new instrument is a great asset to your group."

"We have achieved higher standard in characterization of lignin polymers using the SEC-MALS instrument. The outstanding service and technical support from Wyatt was one of the critical factor in that."

"Easy to use once you acquire enough training. I'm unfamiliar with the value for money statistic because I did not purchase it. The after sales care is some of the best I've ever had."

"The instrument is very easy to use and adaptable to a multitude of solvents and polymeric materials. The service of the instrument is top quality in both the instrumental advice and consultation about methodology. A great staff of knowledgeable people with a true desire to assist with guidance."

"I enjoy using the Dawn TREOS for my molecule weight analysis. I look forward to using the Nanostar DLS to quantify the binding interactions of the complex. Overall, I am looking forward to publishing my results and using the technologies of Wyatt. "

"All great - easy to use and gives great results. Wyatt people are very helpful."

"High quality, reproducible results, Easy to use Astra software, Great customer service and support,"

"The results from this instrument are reproducible, specific, and it is incredibly easy to use and maintain."

"The Heleos II MALS was well designed and robust. Customer service representatives are courteous and knowledgeable."

"Great product. Very good value for money. Wyatt Technology is consistently offering high quality products."

"The instrument does much more than we need for nearly all of our applications. It was daunting to create a protocol for our needs, but once we established a simplified method that matches our simple requirements it has been easy to show others how to use it in the same manner."

"Although in the upper price range of light scattering equipment on the market the equipment is more than worth the money. Quality of the equipment, reproducibility of results and effectiveness are great. The support is above average."

"The MALS along with the Viscostar II and T-rex have given our lab a powerful tool in characterization of unknown polymers. We have been successfully characterizing raw materials, unknowns, and problem batches for our company for over a year now. We now can also get accurate polymer MW and distribution data where before we could only compare to standards (usually a dissimilar polystyrene). The system does require a relatively skilled person to perform analyses, but Wyatt also paid for training! Every time I have needed any help the highly trained Wyatt staff have answered questions very quickly. The support is wonderful."

"A lot of noise in the signal and it is not easy to have a full window of the recording on the instrument itself. It is possible with the software but it will be useful to have it from the window of the instrument. I don't like the way to select the different options in the instrument."

"This detector is very effective for the analysis of water-soluble polymers with very high molecular weights (and very low also). The results (Mn, Mw, D, Rg) are reproducible and of high quality. When there is a problem, it comes from very rarely from this detector but from SEC columns, pumps...!"

"We find the equipment to be highly reliable. We have had many issues with the DRI (T-Rex) and the Viscometer requiring service but the MALS is working very well."

"We use the instrument intermittently primarily because we are not able to control Astra via our enterprise Empower software. However, I would like to use it on a day-to-day basis and expand its use beyond ADC analysis i.e. nano-particle characterization"

"Easy to use equipment. Always can rely on help from Manufacturer network. Reliable results and very good reproducibility. The set-up works 24/7/365."

"The instrument is fantastic and sensitive. It gave us results that we just could not get any other way. We've since had outside users characterize some very exotic samples on the Heleos II and they are very impressed with the molecular weight and size characterizations. This is definitely the top of the line in light scattering detectors."

"Very easy to use. When used with size exclusion chromatography gives absolute Mw and purity of proteins. I use it to follow the effect of ligands on protein association. "

"We use it for PEGylated protein analysis. It is a complete system with Agilent SEC and Optilab T-rEX. There is a steep learning curve for understanding the principles and mastering the data processing, however for us this has been one of the most reliable instrument providing accurate data. It has also been quite precise for method development. Extremely high sensitivity and complexity of the instrument make it highly vulnerable to slightest lack of care and maintenance. However, Wyatt service has been extremely helpful with providing useful information on use and maintenance of their products. We have also found the Wyatt online library to be of great help for understanding the applicability of the instrument for applications beyond what we are using it for."

"We chose the Wyatt MALS/RI instrument for three reasons: the ability to determine the absolute molecular weight of macromolecule, to assess monodispersity or conformational heterogeneity, and to be able to estimate the composition of protein conjugates. This has allowed us to routinely characterize the composition of protein detergent complexes which would otherwise been difficult to assess otherwise. The measurement protocols are easy to follow and greatly simplify protein characterization. Results are presented in an informative manner. "

"The system is easy to use and provide reliable results. The challenge with after-sales service is the lack of competent service engineers in the Kingdom of Saudi Arabia for maintenance and service programs. Wyatt should engage a local vendor with trained engineers to assist customers in both preventative and corrective maintenance. Supply of parts and consumables for normal optimum running of equipment should also be contracted to local vendors."

"The product itself is quite robust and easy to use. The software is a little cumbersome and could be more intuitive. Otherwise, a great product."

"SEC-MALLS is a powerful technique that is indispensable for certain applications, monitoring glyco-conjugates being one of those applications. Without SECMALLS we would be still be doing gravity columns and colorimetric analysis of fractions. I just wish we had less repairs with these instruments, we have averaged 2-3 weeks of instrument downtime a year. (n =9, 6 years)"

"This is an excellent instrument, but without a doubt one of the best companies that I have worked with in over 30 years. They care not only about the sale, but they want to be sure that the client gets good results. Highly recommended!"

"Easy to use instrument and software, Good technical support, but difficult to repair at work place "

"I'm very happy with this instrument. We obtain high-quality results and the interface, analysis software and instrument capabilities are significantly better than many other light scattering instruments I have used. Also, the quality of technical assistance provided by Wyatt staff is absolutely amazing. Probably the best customer service I have ever received from an equipment manufacturer."

"The equipment produces high quality data that is reproducible and invaluable to our development of biologics. The training on how to run the instrumentation is top quality provided by scientists that truly care that you understand the related applications performed by this instrument. The staff at Wyatt are always eager to help in any way that they can whenever I have a question about maintenance, data analysis and troubleshooting. Some of the applications that can be performed with the MALS instrument can be complex. It is wonderful to know that there is always someone there to help me understand both the science and the how-to's of running an experiment."