Products & ReviewLife Sciences

BHQ™ Probes

Name: BHQ™ Probes
Rating: 4.51 (7 reviews)

LGC Biosearch TechnologiesAvailable: Worldwide

Dual-labelled probes featuring the original BHQ dye technologies

4.5/5.0

|7 Reviews|Write your own Review

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Place your order directly with the manufacturer.

Reviews

Average Rating 4.5

|7Scientists have reviewed this product

Ease of Use

After Sales Service

Value for Money

Write your own review

decent results

Average Rating 4.7

Application Area:

Analyze mRNA expression in brain tissues and cell cultures of Drosophila melanogaster

Probes are of high quality and very easy to use. I would give it 5 stars if it could be less expensive.

Review Date: 3 Aug 2017 | LGC Biosearch Technologies

it is very good company and I would recommended to other colleagues.

Average Rating 4.3

Application Area:

analyzed swab, whole blood as well as tissues samples

It is easy to you, meet our requirement. Delivery on time to commitment. Pre order and quotation are very fast.

Review Date: 1 Aug 2017 | LGC Biosearch Technologies

Competitive prices and fast shipping.

Average Rating 4.3

Application Area:

Diagnostics

As always, the primers and probes worked well as intended. I recently took over ordering for my lab and they made ordering as easy as possible and made sure to follow up with me when I failed to submit the complete order. Arrived timely and at a competitive price compared to another vender we use for the same reagents.

Review Date: 1 Aug 2017 | LGC Biosearch Technologies

Overall good services and products

Average Rating 4.3

Application Area:

Recombinase Polymerase Assay

Products were good and packing was also okay but delivery takes time.

Review Date: 1 Aug 2017 | LGC Biosearch Technologies

So far, so good!

Average Rating 4.0

Application Area:

Genotyping

Product is value for money for our application. Quality is reproducible. I do notice that the colour intensity of resuspended probes varies from batch to batch although I made sure to add the right amount of resuspending bufffer. I would presume that it might have to do with the concentration (OD) of probes among batches? For our application, fluorescence values are interpreted in LOG form, so the effects are negligible.

Review Date: 1 Aug 2017 | LGC Biosearch Technologies

The LGC Biosearch Technologies TaqMan Chemistry probes are a part of ever day work!

Average Rating 5.0

Application Area:

Quantitative real time PCR

The LGC Biosearch Technologies TaqMan Chemistry probes for real time quantitative PCR are of very good quality, suitable for multiplex PCR reactions, and always give precise and reproducible results. We rely a lot on the LGC Biosearch Technologies probes which allow us perform high quality assays at a good price

Review Date: 31 Jul 2017 | LGC Biosearch Technologies

Great results! Probe turnaround time fastest yet.

Average Rating 5.0

Application Area:

qPCR for Zika Virus detection in various murine mouse tissues.

A high-quality probe that provides reproducible results. Commonly used in our lab and commonly requested by our collaborators down in Nicaragua.

Review Date: 31 Jul 2017 | LGC Biosearch Technologies

Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence.

Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences.

Application eBookLife Sciences

Top tips for robust qPCR results

Reproducibility issues are a common pain point experienced by scientists carrying out quantitative polymerase chain reaction (qPCR), or real-time PCR, with challenges being linked to suboptimal design, probe selection, or workflow execution. The inconsistencies impact not only data quality, but also wasted time, lost samples and delayed research milestones.

A successful qPCR experiment relies on a well‑designed workflow, from high‑quality sample preparation to optimized reagents, primers, and probes, supported by thoughtful assay design that avoids issues like nonspecific binding or inefficient amplification. Expert probe‑selection strategies enhance specificity, especially in multiplexing, while effective troubleshooting helps diagnose common problems such as poor efficiency, primer‑dimers, or high variability. By applying key optimization techniques, researchers can achieve sensitive, accurate, and reproducible qPCR results.

Download this SelectScience guide to explore:

How to overcome challenges in qPCR assay design
A guide to selecting the right qPCR probe
The best way to troubleshoot your qPCR
Tips for qPCR optimization and validation

Resource details:

Document type: SelectScience guide
Page count: 49
Read time: 69 mins
Edition: 1st

9 Feb 2026

Application eBookClinical Diagnostics

Simplifying the journey from molecular diagnostic assay development to commercialization

Molecular diagnostics (MDx) is rapidly advancing, with a growing array of DNA- and RNA-based tests now available to physicians, pharmacists, geneticists, research scientists, and other healthcare professionals.

As labs work with human and microbial genes, test developers must comply with clinical guidelines and regulations. Both established companies and start-ups face challenges, including meeting regulatory standards, ensuring quality, and managing supply chain issues. Early planning and robust partnerships are key to overcoming these obstacles.

This essential guide explores these challenges through detailed case studies. It covers everything from early development and assay formulation, to supply chain security, offering expert insights on successfully bringing PCR, next-generation sequencing (NGS), and other nucleic acid-based diagnostic tests to market.

30 Jul 2024

VideoClinical Diagnostics

The future of diagnostics: How innovators in molecular techniques are shaping healthcare

From enzymatics to informatics, Hillary Sullivan, Vice President of R&D at LGC Biosearch Technologies, shares her thoughts on some of the latest advancements in molecular diagnostics. With insights from successful partnerships in healthcare, Sullivan provides her perspective on what the most promising areas of scientific advancements might hold for this rapidly developing field in the next 25 years.

30 Jul 2023

VideoDrug discovery > Target Discovery

7 innovative qPCR probe types explained

This comprehensive walkthrough animation outlines the latest developments in probe technologies in the qPCR field, highlighting the mechanisms of seven different real-time PCR probe and primer formats:

Dual-labelled hydrolysis probes [00:19]
BHQnova™ Probes [01:17]
Minor groove binder (MGB) probes [01:49]
Locked nucleic acid (LNA) probes [02:31]
BHQplus™ Probes [03:15]
Molecular Beacons [04:45]
Scorpions™ Primers [06:00]

4 Jun 2023

Editorial ArticleClinical Diagnostics

Decentralizing healthcare through point-of-care diagnostics

Portable testing platforms are set to drive precision medicine and community health into 2024, so when will they see a more widespread adoption amongst healthcare providers and individuals alike?

13 Dec 2023

Editorial ArticleClinical Diagnostics

LAMP-based diagnostics: Rapid and truly portable molecular testing at the point-of-need

Discover how a multi-disciplinary team located at Imperial College London created a novel portable diagnostic platform and delivered a rapid and accurate 5-in-1 respiratory pathogen detection test panel

12 Jan 2023

Editorial ArticleClinical Diagnostics

A revolution in diagnostics – The race for five-minute point-of-care PCR testing

Learn how UK-based diagnostics company, LEX Diagnostics, is working with LGC Biosearch Technologies to precipitate a step-change in PCR usability and speed

18 Jul 2022

Editorial ArticleLife Sciences

8 game-changing resources for infectious disease research

Explore the solutions, expert insights, and newest technologies that are driving COVID-19 and infectious disease research

23 Mar 2022

Editorial ArticleLife Sciences

Combating antibiotic-resistant sexually transmitted infections with molecular diagnostics

Explore how a partnership between a lead researcher in sexual health and a molecular diagnostics innovator helped to overcome the therapeutic hurdles that come with antibiotic resistance

12 Jan 2022

Editorial ArticleClinical Diagnostics

Transition to molecular diagnostics: How agricultural expertise has helped to keep COVID-19 positive rates low

Making the most of agrigenomic expertise and existing technologies to enable a rapid response to the pandemic

19 Nov 2021

Product Overview

BHQ™ Probes

LGC Biosearch Technologies

4.5/5.0

|7 Reviews

Buy Now

Links

LGC Biosearch Technologies Manufacturer Website

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