Top tips for robust qPCR results

Reproducibility issues are a common pain point experienced by scientists carrying out quantitative polymerase chain reaction (qPCR), or real-time PCR, with challenges being linked to suboptimal design, probe selection, or workflow execution. The inconsistencies impact not only data quality, but also wasted time, lost samples and delayed research milestones.

A successful qPCR experiment relies on a well‑designed workflow, from high‑quality sample preparation to optimized reagents, primers, and probes, supported by thoughtful assay design that avoids issues like nonspecific binding or inefficient amplification. Expert probe‑selection strategies enhance specificity, especially in multiplexing, while effective troubleshooting helps diagnose common problems such as poor efficiency, primer‑dimers, or high variability. By applying key optimization techniques, researchers can achieve sensitive, accurate, and reproducible qPCR results.

Download this SelectScience guide to explore:

• How to overcome challenges in qPCR assay design
• A guide to selecting the right qPCR probe
• The best way to troubleshoot your qPCR
• Tips for qPCR optimization and validation

Resource details:

• Document type: SelectScience guide
• Page count: 49
• Read time: 69 mins
• Edition: 1st