ACQUITY UPLC System

In this application note, Waters™ describes how the Automation Portal enhances laboratory efficiency, throughput and consistency by automating the ACQUITY™ and Arc™ UPLC™, UHPLC, and HPLC Systems. It accomplishes this by streamlining the liquid chromatography (LC) workflow, specifically through the use of robotic arms to manage physical sample transfers into ACQUITY and Arc Sample Managers, thus reducing the potential for analyst errors. Waters emphasizes the Portal's versatility, as it can work with scheduling software for managing laboratory tasks and can integrate with different lab environments and preferred integration partners.

Due to rising concerns about the long-term impacts of per- and polyfluoroalkyl substances (PFAS) on human health, many agencies across the globe have been studying the occurrence of PFAS in food. Waters™ previously developed a method for the determination of PFAS in complex food commodities such as fish, meat, edible offal, and eggs which requires a comprehensive sample extraction and clean-up. This application brief shows the successful evaluation of the performance of this method by interlaboratory study. It is based upon an alkaline extraction, sample clean-up using Oasis™ WAX for PFAS Analysis SPE cartridge and determination using ACQUITY™ UPLC™ System fitted with the PFAS Analysis Kit, Isolator Column and Xevo™ TQ-XS Tandem Quadrupole Mass Spectrometer. A reference material of fish containing PFHxS, PFOS, PFOA, and PFNA was sent to seven Waters laboratories, along with standard solutions containing native PFAS compounds and isotopically labelled analogues. For the four PFAS analytes, trueness of the method was determined to be within the range of 102 to 121%. Repeatability within laboratories was <20% and between laboratory reproducibility ≤30%.

Ensuring high-quality oligonucleotides, either in diagnostic applications or as therapeutic entities, relies on robust analytical methods. In this application note, Waters presents some critical column and chemistry best practices for performing successful oligonucleotide analysis by ion-pair reversed-phase (IP-RP) liquid chromatography.

In this application note, Waters demonstrates that, when using the new PREMIER columns, which utilize MaxPeak High Performance Surface (HPS) Technology, dramatic improvements in peak shape for these metal-sensitive analytes were observed.

Despite the widespread practice of exact-mass mass spectrometry (MS) analysis in the biotherapeutics development processes, experienced MS users are typically required to manage the instrument operation, data processing, and interpretation. In this study, Waters reports on the development of a new high-performance bench-top orthogonal acceleration time-of-flight (oa-TOF) LC-MS system (BioAccord) designed with simplified and optimized operation modes to deliver automated, accurate, and reproducible mass measurements for proteins, peptides, and released glycans.

This application note demonstrates how the BioAccord can increase confidence and productivity in released glycan analysis for the development of biosimilars.

In this application note, Waters demonstrates an improved N-glycan preparation on the Andrew Alliance pipetting robot with automated vacuum controls.

In this application note, Waters perform strong cation exchange ultra-performance liquid chromatography coupled to UV with a non-volatile salt/pH gradient as well as LC-UV-MS using volatile MS-compatible salts. In-depth analyses were performed on AJICAP mAb and ADC charge variants to allow for effective resolution and characterization of charge variants throughout the ADC manufacturing process.

Ion exchange chromatography (IEX) is a method of choice for the analysis of charge heterogeneity encountered with biotherapeutic drug candidates. In this study, Waters conducted MS-compatible IEX separations combined with a new small footprint benchtop time-of-flight (TOF) MS instrument and applied to a case study on identifying the charge variants formed upon forced degradation.

This poster demonstrates a compact, simple and sensitive HRMS platform with improved data quality evaluated for intact level mAb quantitation in plasma.

Monday, November 3, at 16:00 GMT | 17:00 CET | 11:00 EST | 08:00 PST

Many chromatographers face challenges with polar analyte separation, due to their strong interactions with the stationary phase or poor retention in reversed-phase systems. These challenges often result in suboptimal resolution, peak tailing, or co-elution, often leading to time-consuming re-analysis and column chemistry adjustments.

Join Dr. Chris Collins, Waters Corporation, as he reveals the mechanisms behind polar analyte retention and guides you in how to select the perfect column for seamless separations.

Key learning objectives:

• Understand polar compound retention in reversed-phase HPLC and explore mixed-mode and HILIC alternatives.
• Explore the underlying principles and challenges of retaining and separating polar analytes.

Who should attend?

• Chromatographers who need support in retaining and separating polar analytes in the pharmaceutical, environmental and material science industries.

Certificate of attendance
If you attend the live webinar, you will automatically receive a certificate of attendance, including a learning outcomes summary, for continuing education purposes.

If you view the on-demand webinar, you can request a certificate of attendance by emailing editor@selectscience.net.

Understand the value of accurate and precise measurement in biomedical research: Professor of Medicine, Dr. Stokes Peebles, and Research Associate Professor of Medicine, Dr. Ginger Milne, from Vanderbilt University School of Medicine, discuss how mass spectrometry provides analytical rigor not achievable with immunoaffinity technologies.

Liquid chromatography tandem mass spectrometry is advancing the analysis of clinical samples and helps improve efficiency in patient sample processing. Learn how Waters Xevo® TQ-S and ACQUITY UPLC® systems are being used at UC San Diego to develop new methods for accurate quantitation of analytes in a short amount of time.

Mass spectrometry is now a reliable and user-friendly technique. The current data analysis software available is unable to keep up with the volume of data produced in industrial laboratories. The simplification and acceleration of analyses is the step forward needed to see mass spectrometry become a routine technique in industry.

As a leading clinical LC-MS/MS solutions partner, Waters is a trusted provider of in vitro diagnostic medical devices, consumables, informatics, and support services for high performance LC-MS/MS systems. Learn how and why University Hospital of South Manchester has introduced Waters LC-MS/MS IVD instrumentation into their busy clinical laboratory.

Watch this video to learn how to easily transfer methods from existing instrumentation to the Waters ACQUITY and Alliance systems. For more information, click on the website link below.

Hear Art Caputo, President of Waters Corporation, and Ian King, Vice President of Separations Technology, celebrate Waters’ success as the winner of Product of the Year 2015 for the ACQUITY UPLC System at the Reviewers’ Choice Awards. The ACQUITY UPLC System was the most reviewed product of the year by the global SelectScience community. The award was presented at the exclusive Scientists’ Choice Awards ceremony at Pittcon 2105 in New Orleans, LA.

Discover how the mass spectrometry analysis is used to help Bruno manage his hydration and glucose levels, to ensure he performs at the optimum level and recovers properly.

Watch this video to learn how mass spectrometry is used for metabolomics research. Elizabeth Want, PhD., Imperial College, explains her research involving the development and optimization of mass spectroscopy based metabolite profiling. Solutions provided by Waters have allowed for higher throughput, increased sensitivity, and less time required.

Watch Prof Fabio Gozzo, Chemistry Institute at the University of Campinas, explain how he develops and applies different mass spectrometry techniques to understand protein structures and protein-protein interactions. By understanding how molecules interact with each other, and how structure plays a role in these interactions, Prof Gozzo’s team are identifying cell signalling pathways that can be potentially influenced, with many applications such as in cardiac hypertrophy. SelectScience Interview for ‘Science in Brazil’ Special Feature.

As India starts to tackle PFAS, CSIR-IITR is leading the charge in developing detection methods using Waters’ advanced technologies

The announcement at Pittcon 2024 highlights the integration of essential instruments for large molecule characterization

Dr. Thomas H. Walter, of Waters Corporation, looks back at the trials, tribulations, and triumphs that paved the way to today’s advanced separation technologies

Waters’ newest tandem quadrupole mass spectrometer is up to 15X more sensitive for analyzing negatively ionized compounds

Watch this on-demand webinar to learn how to perform untargeted/semi-targeted metabolomic studies to discover novel biomarkers by mass spectrometry

The Incucyte Live-Cell Analysis System from Sartorius becomes the fifth product in history to be awarded a Platinum Seal of Quality

Experts Dr. Paula Hong and Jennifer Simeone review how each autosampler aliquots and delivers the sample and discuss their differences

In this popular on-demand webinar, learn more about which applications may benefit from a particular pump and how to select an LC system based on your application needs

Dr. Pauline Rudd explains why glycosylation matters, how it is measured using tandem analytical methods, and the potential that glycobiology has in therapeutics