SPRIselect Reagent Kit for DNA Size Selection
SPRIselect is a SPRI-based chemistry that speeds and simplifies nucleic acid size selection for fragment library preparation for Next Generation sequencing.
The supplier does not provide quotations for this product through SelectScience. You can search for similar products in our Product Directory.
Great, but hard to get!
DNA fragmentation
I used the SPRI beads to generate fragmented DNA for a study that we were doing and I enjoyed the performance of these beads. Also, I like that these can be stored at room temperature unlike some of the other brands that I have which have to be kept at 4C. The results so far are really consistent operator to operator. However, at the time of purchase, it did take more than a couple weeks for them to actually arrive which was not ideal.
Review Date: 18 Jun 2021 | Beckman Coulter Life Sciences
SPRIselect is a SPRI-based chemistry that speeds and simplifies nucleic acid size selection for fragment library preparation for Next Generation sequencing. In this process, size selection is required to produce a uniform distribution of fragments around an average size. Using SPRIselect, the size distribution can be adjusted to suit the application and platform used. The process can be scaled for low to high throughput workflows. Used manually or automated on a liquid handling system such as the Biomek Laboratory Automation Workstation, SPRIselect will provide rapid and consistent size selection suitable for most applications.
Features:
- Tunable from 150 to 800 base pairs
- Efficient mag bead technology eliminates the need for gels, chips or special instrumentation
- Easily adjust for specific application and sequencer
- Consistent results, reproducible sizing between runs and reagent lots
- Scalable from manual to automated for highthroughput processing in 96-well plates
- Proven proprietary technology is already the gold standard for purification steps in Next Gen Sequencing (NGS) sample prep
Accelerate green chemistry with smarter synthetic biology
Synthetic biology is driving a new era in sustainable science—enabling researchers to design microorganisms for the efficient production of environmentally friendly materials. Whether you're engineering microbial strains or optimizing growth conditions, minimizing waste and maximizing process efficiency are core goals of any green chemistry workflow.
Download this eBook to:
- Miniaturize and automate strain development — cut reagent use and reduce plastic waste
- Improve strain growth and optimization — minimize costly trial-and-error steps
- Speed up process development — reduce timelines without sacrificing data quality
Explore real-world case studies and practical tools that support sustainable innovation—from DNA purification to high-throughput bioprocessing. Learn how automation, precision liquid handling, and scalable workflows can help you bring green chemistry principles to life in your lab.
Resource details:
- Document type: SelectScience guide
- Page count: 91
- Read time: 2.5 hours
- Edition: 1st
RNA-Seq Library Preparation Using SPRIworks Fragment Library System I
SPRIworks Fragment Library System I is a fully automated fragment library construction system for the Illumina Genome Analyzer. This application note demonstrates the capability of the SPRIworks system to create high quality RNA-Seq libraries using cDNA generated with the Illumina recommended cDNA synthesis procedure. The SPRIworks system minimizes hands on time while increasing the throughput for library construction by providing a walk-away solution for constructing up to 10 libraries at a time.
Highly Efficient Micro RNA Enrichment Procedure Using Solid Phase Reverse Immobilization Magnetic Bead Technology
This technical note describes the use of Beckman Coulter‘s SPRIselect reagent kit for miRNA enrichment. The
Solid Phase Reverse Immobilization (SPRI) magnetic bead method is a nucleic acid purification procedure that does not require centrifugation or filtration steps. This method is simple, rapid, automation friendly and produces high quality samples.
Automation of NGS Sample Size Selection Using SPRIselect on the Biomek 4000 Laboratory Automation Workstation
To streamline the NGS sample preparation process, Beckman Coulter launched the SPRIselect Reagent Kit to allow NGS sample size selection to be automated on Biomek liquid handling platforms for high throughput and high efficiency sample preparations. The data presented here demonstrates the automation of the SPRIselect Kit on the Biomek 4000 Workstation. The demonstrated method will prepare 96 samples in 96-well plate format for small to medium throughput laboratories.
High Throughput miRNA Purification from FFPE, Cell and Tissue Samples Using Solid Phase Reverse Immobilization (SPRI) Technology and Biomek FXp Liquid Handler
This poster describes an efficient and robust miRNA extraction method using the Agencourt SPRI (Solid Phase Reverse Immobilization) technology and Biomek automation. Three extraction methodologies described are (1) miRNA extracted and purified from FFPE samples using the Agencourt FormaPure Kit, (2) miRNA extracted and purified from cell culture using the Agencourt RNAdvance Cell v2 kit and (3) miRNA extracted and purified from tissue using the Agencourt RNAdvance Tissue kit.
Automated Size Selection using SPRIselect Beads and the Biomek FX Liquid Handler
This application note describes the automation of the Size Selection using SPRIselect beads on the Biomek FX Liquid Handler.
Gel-Free Size Selection Using SPRIselect For Next Generation Sequencing
Gel size selection for Next Generation Sequencing (NGS) fragment library preparation has been the standard process for achieving size selection. Aside from being tedious, gel size selection creates additional preparation time for scientists forcing them to purify the desired fragment sizes from the gel creating a process that can only be low throughput. In this poster read how SPRIselect, from Beckman Coulter, helps to alleviate this problem.
Workflow Challenges. Solved.
iRepertoire in Huntsville, Alabama provides products and services supporting the study of the immune repertoire. The company’s patented amplicon rescued multiplex arm-PCR technology requires optimization of many intermediate steps to achieve a precision result. Optimization of these steps, and the final outcome, are greatly enhanced when using Agencourt SPRIselect for size selection, and to concentrate, clean-up and rescue RNA and cDNA throughout the process.
In this video, research scientist Katie Limbaugh introduces us to iRepertoire and how SPRIselect helped improve overall workflow results.
miRNA Enrichment and RNA Size Selection Using SPRI Paramagnetic Bead Technology
This presentation describes a flexible method for miRNA enrichment and RNA size selection using solid phase reversible immunobilization (SPRI) paramagnetic bead technology.
