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High Throughput miRNA Purification from FFPE, Cell and Tissue Samples Using Solid Phase Reverse Immobilization (SPRI) Technology and Biomek FXp Liquid Handler

Beckman Coulter Diagnostics
6 Feb 2015

This poster describes an efficient and robust miRNA extraction method using the Agencourt SPRI (Solid Phase Reverse Immobilization) technology and Biomek automation. Three extraction methodologies described are (1) miRNA extracted and purified from FFPE samples using the Agencourt FormaPure Kit, (2) miRNA extracted and purified from cell culture using the Agencourt RNAdvance Cell v2 kit and (3) miRNA extracted and purified from tissue using the Agencourt RNAdvance Tissue kit.

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RNAdvance Cell v2 kit

Beckman Coulter Life Sciences

The Agencourt RNAdvance Cell v2 kit is based on Solid Phase Reversible Immobilization (SPRI) paramagnetic bead technology, known for its ability to consistently deliver pure nucleic acids of the highest quality and yield.

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RNAdvance Tissue

Beckman Coulter Life Sciences

The Agencourt RNAdvance Tissue total RNA extraction technology in combination with Beckman Coulter automation offers researchers a true walk away solution for consistent recovery of high quality total RNA in a multi-well format.

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DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.BiomarkersBiomarkers are biological markers which can be measured and evaluated to indicate a biological state. The use of biomarkers in research and diagnosis can indicate a normal or disease state or drug response of cells / tissues. Biomarkers include genetic markers, cell surface markers such as antigens, antibodies or receptors and secreted molecules such as cytokines. An assay system is required for identification of biomarkers. :Automated Liquid HandlingAutomated liquid handling equipment is used to mix, dilute and dispense allotted volumes of liquid into microplates and other vessels automatically. The robotic, liquid handling arms can dispense from single channel to 3456 multichannel as well as operate nanoliter dispensing, enabling high throughput of samples. Find the best automated liquid handlers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.FFPEFormalin-fixed paraffin-embedded (FFPE) tissues are commonly used in medical research, particularly in histology and cancer studies. The preservation process allows long-term storage of tissue samples for later analysis. Explore FFPE research products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.miRNA

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High Throughput miRNA Purification from FFPE, Cell and Tissue Samples Using Solid Phase Reverse Immobilization (SPRI) Technology and Biomek FXp Liquid Handler