Products & ReviewLife Sciences

RNAdvance Cell v2 kit

The Agencourt RNAdvance Cell v2 kit is based on Solid Phase Reversible Immobilization (SPRI) paramagnetic bead technology, known for its ability to consistently deliver pure nucleic acids of the highest quality and yield.

Beckman Coulter Life Sciences

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Description

The Agencourt RNAdvance Cell v2 kit is based on Solid Phase Reversible Immobilization (SPRI) paramagnetic bead technology, known for its ability to consistently deliver pure nucleic acids of the highest quality and yield. This system reliably delivers high RNA recovery and purity without the need for filtration or centrifugation. The Agencourt RNAdvance Cell v2 chemistry is an automation friendly process for isolating total RNA for utilization in microarray and real time qPCR applications. With flexibility from manual to fully automated 96-well plate formats, the Agencourt SPRI paramagnetic bead-based technology enables efficient removal of contaminants without the need for filtration or centrifugation. Agencourt RNAdvance Cell v2 produces higher recovery, better quality, and more consistent results compared to other available RNA isolation and purification technologies with a walk-away automation solution.

Features:

  • Extraction and purification of high quality total RNA from cultured cells in a 96-well format
  • Efficient removal of genomic DNA and other contaminants
  • No centrifugation or vacuum filtration required Ideal for gene expression applications
  • Scalable: Supports manual processing and full automation methods on Beckman Coulter Biomek NXP and FXP laboratory automation workstations
  • Throughput: Two 96-well plates in approximately 2 hours and 15 minutes on the Beckman Coulter Biomek FXP


Application NoteLife Sciences

MicroRNA Extraction from Purified Exosomes Using Beckman’s Agencourt RNAdvance Cell v2 Kits and the Biomek NXP MC Platform

Tumor and normal cells may contain different subsets of microRNA expression profiles which can be found in exosomes. Therefore, monitoring the exosomal miRNAs can be used for early cancer screening and detection. This application note describes how the Agencourt RNAdvance Cell v2 kit from Beckman can be used to extract miRNA for this reason.


Scientific PosterLife Sciences

High Throughput miRNA Purification from FFPE, Cell and Tissue Samples Using Solid Phase Reverse Immobilization (SPRI) Technology and Biomek FXp Liquid Handler

This poster describes an efficient and robust miRNA extraction method using the Agencourt SPRI (Solid Phase Reverse Immobilization) technology and Biomek automation. Three extraction methodologies described are (1) miRNA extracted and purified from FFPE samples using the Agencourt FormaPure Kit, (2) miRNA extracted and purified from cell culture using the Agencourt RNAdvance Cell v2 kit and (3) miRNA extracted and purified from tissue using the Agencourt RNAdvance Tissue kit.


Application NoteLife Sciences

Highly-efficient miRNA Isolation using the Agencourt FormaPure and RNAdvance Cell v2 Kits and Biomek Automated Extraction Methods

This application note describes an efficient and robust miRNA extraction method using Agencourt SPRI (Solid Phase Reverse Immobilization) technology and Biomek automation. The SPRI procedure is an easy, rapid, high yield, and automation-friendly nucleic acid purification method that does not require organic solvents, centrifugation or filtration steps. This method uses carboxyl-coated magnetic particles that reversibly bind nucleic acids in the presence of binding buffers and crowding reagents.

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