Milo™
Milo™ lets you identify cellular subpopulations that aren't visible in traditional Western Blots, so you can study distinct cell types in your heterogeneous sample.
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Milo™ is the world's first Single-Cell Western platform. He measures protein expression in thousands of single cells in a single run so you can profile heterogeneity in your samples. Just load your cell suspension and the scWest chip captures ~1,000 single-cells. Milo then does a fast, 1 minute SDS-PAGE separation on each single-cell lysate on-chip. Then just probe with your favorite conventional Western antibodies to measure up to 4 proteins per cell simultaneously. Use Single-Cell Westerns to unlock the single-cell proteome and measure more of the proteome than is possible with any other single-cell technique.
Protein Expression Heterogeneity with Milo, the First Single-Cell Western System
More and more high-profile publications are using single-cell analysis techniques to reveal variability in cellular response to a drug or stimulus. Single-cell protein expression information is critical when you need to understand the fundamental composition and behavior of complex biological samples. In this application note, the features of Milo are discussed, a Single-Cell Western blotting platform.
