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Protein Expression Heterogeneity with Milo, the First Single-Cell Western System
23 Feb 2018More and more high-profile publications are using single-cell analysis techniques to reveal variability in cellular response to a drug or stimulus. Single-cell protein expression information is critical when you need to understand the fundamental composition and behavior of complex biological samples. In this application note, the features of Milo are discussed, a Single-Cell Western blotting platform.
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Bio-TechneMilo™ lets you identify cellular subpopulations that aren't visible in traditional Western Blots, so you can study distinct cell types in your heterogeneous sample.
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Western BlottingWestern blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.VoltammetryVoltammetry is a sensitive analytical technique for elemental detection. In a voltammeter the current is measured in the system whilst the voltage is varied. Usually 3 metal electrodes are required including the reference, auxiliary and working electrodes. In polarography, a subclass of voltammetry, the working electrode used is a dropping mercury electrode (DME). The 2 main types of voltammetry are cyclic and linear sweep voltammetry.