Kinetex Core-Shell HPLC/UHPLC Columns by Phenomenex Inc

"I was looking for a column that has increased retention for some polar biomolecules which I need to develop a separation for. Tried other similar column chemistries from other manufacturers but the peak tailing was significant on the other columns. The Kinetex Polar C18 column gave good separation between my polar biomolecules of interest with minimal peak tailing. Great column to use for my application."

"Excellent technology that gives proven results. Very important tool for scientists who still work with normal LCs, as by using this column technology one can establish methods almost equal to UPLC."

"We used it for method development for drug development all the time. Our go to. "

"The Kinetex columns with Core-Shell technology were introduced about a decade back, but still have good use. Especially for laboratories which are not equipped with high pressure systems (UPLC), these types of columns are "Must Have" accessories for an analyst. Great results, great robustness and best fit to cost and support from manufacturer."

"I've had a very good experience with using these columns. They come with a wider pH range with high reproducibility, lot to lot. They also have the capacity for good peak shape and good separation."

"Kinetex core-shell columns are very extensively used in our lab. The column has a good selection for the different stationary phase. Kinetec column offers less back pressure and good separation of closely eluting peaks. The column also has good loading capacity for the sample. This column gives good applicability for HPLC, UPLC and prep. HPLC. It finds the application from small molecule to large molecule pharmaceuticals. The column shows reproducible results and rugged performance."

"This column provided the best peak shape of the C-18 columns evaluated. The samples are extracts thought to contain long chain carboxylic acids. It seems like the column will last a long time. The flushing time for storing is not unreasonably long."

"The Kinetex analytical column uses less solvent, gives results faster and helps save time and money, it also helps the environment by polluting less with less solvents. Great column with high resolution."

"Good product for estimation of inhalation products in biological matrix. I used this column for analysis of more than 200 subjects. No issue in terms of peak shape and consistency in chromatography."

"Very good product (revolutionary in chromatography), changed the life of chromatographers who still works on 2D-HPLC systems where high pressure is always a concern. Using traditional columns gives poor separations and long run times. Kinetex Core-Shell chemistry changed the phenomenon, enabled analysts to work on HPLC with almost the same results as UPLC."

"A product that gives very clean data and is very reproducible. The lifetime of the product is very good. Product is afforable and very accessible."

"It has very good selectivity and provides very sharp peaks with very low tailing factor. Compared to other similar vendors' C-18 columns, Kinetex EVO C18 has the best UHPLC column performance, stability, reliability, and repeatability."

"The product was lucrative and very useful for the development phase of API molecules, specially for the laboratories which are still not equipped with FAST LC or UPLCs. A wonderful product, I simply loved using it."

"This column is very versatile.  I'm currently using it on our LC's for multiple methods. Very robust column.  "

"Great universal column for quantification and qualification"

"Great column and easy to maintain. Results are consistent with little variation in peak area and retention times."

"I used this column for method development for pesticides in juice samples. Good peak shapes and chromatographic profiles for most of my peaks of interest even when matrix effects are present in the samples. Good lifetime and column robustness as well."

"We obtain many savings with new methods developed with this product."

"The column is easy to install, and great value for money."

"We have been using Phenomenex kinetex columns for over a year and we are very happy with the column reproducibility, & stability"

"Very Robust column. It can tolerate high pressures and produces high resolution. Column can take a licking and keep on ticking. I have used the column for multiple methods and applications"

"Reproducible results with a high accuracy. easy to clean and wash off impurities."

"The customer service is incredible and the column has made the run time shorter and the peaks sharper. "

"The column was suitable for separating a sample of small molecules containing an isomer."

"Very useful column for a variety of applications, most of the time allows enhanced peak chromatography and peak resolution."

"Easy to use and for a good value price/utility, these columns are great to separate compounds otherwise really hard to separate from each other."

"Consistent column performance in UPLC applications at a fair price."

"I prefer Kinetex Core-Shell Columns over other UPLC/UHPLC columns. I have developed quite a variety of small molecule medicinal products using these columns. Usually the peak shapes are good and provides reasonable separation of analytes using simpler mobile phase without adding any modifier. One has to be careful about sample size and injection volume not to overload the column. "

"The core shell columns allow for easy method transfer between UPLC and HPLC systems. Phenomenex offers excellent customer and technical service. I always go to them first when considering new columns."

"The 5um particle in the EVO C18 lends reduced pressures and sharp peaks in lipemic serum samples. I am eager to evaluate the life of the column compared to smaller particle core-shell C18s."

"With our vast range of clients, our quality control lab receives all kind of sample to analyse. This column is often used to analyse a large range of samples. The low cost combined with the great range of pH in which this column can be used saves us a lot of trouble and gives us a great price/utility ratio."

"We use Kinetex 5 micron C18 columns in a synthetic lab for routine reaction monitoring. Most of the time it's a simple dip-dilute-and-shoot sample prep with no specific clean-up. The column survived well and gave excellent results, both in peak shape and sharpness. Some of our samples have very polar and chelating bisphosphonate groups, and the column handled them well. The 5 micron columns give 70~80% of the resolution power of what 2.7 micron columns offer, with significantly lower back pressure. They are well suited for a simple drop-in upgrade for older instruments that cannot handle the high pressure of small particle size columns. The new EVO columns give the same or better peak shape, with significantly wider usable pH range. We haven't used those for long enough to comment on their longevity. But the current column has survived over 500 tissue samples (blend and organic solvent precipitation) without notable degradation. Their customer care and technical support teams are very "

"This HPLC column was of a high value in resolving the impurities and enhanced the peak shape and chromatography for an aromatic compound with several functional groups and several pKa."

"I have worked with this UHPLC Column for several HPLC assays/methods. Great performance in separations and extremely durable. Used in heavy salt buffers for amino acid separations and the performance was exceptional. It has been very easy to care for the column. "

"This column works better than any column I have used for method development. "

"The HILIC kinetex column has been great for separating out those residues that just wont separate by RP HPLC. The biggest challenge has been getting analytes into a solution suitable for HILIC initial conditions. Currently difficult to judge the robustness as it does not see a ton of action. However, the samples it has seen are "problem" plant and animal extracts and it has held up well so far!"

"The column is very sensitive and allows for separation and identification of nano-materials that I would not be able to characterize with other columns. Plus, the offer of 2 columns for the price of 1 was incredible. Thank you."

"The product is easy to use and gave good quality results. For separation of lipids using the RP C18 core shells - we are able to separate the different lipid classes and species and even some isobaric species. The resolution is good and the analyst can even use higher flow rates and still work within relatively low back pressure levels. I would continue to recommend the Kinetex Core-Shell columns. "

"Great retention and selectivity for a panel of steroid hormones. "

"Phenomenex Kinetex F5 Core-Shell Column provides an effective and efficient HPLC analytical solution for separation of phenolic compounds generated from cigarette smoke. It can separate positional isomers such as m-, o-, p- cresols easily. "

"Our Research Team (University of Molise, Campobasso, Italy) has worked for many years on the dietary use of natural extract on the animal welfare and the quality of animal production. To reach new knowledge in this topic, we use several Phenomenex products in order to evaluate the phenolic profile of natural plant extract, to monitor the welfare of animal and to characterize the quality of animal production with different kind of analysis. For the analysis of plant natural extract we have performed a HPLC method using a Kinetex 2.6μ XB – C18 column, reaching very successful results. "

"Low back pressure and great separation performance I suggest this type of column for basic compound"

"I've been working with this company for many years. They have never failed to supply us with the methods and the equipment necessary to help us solve our problems. I would highly recommend this company to all. They always supply us with the information required to make a purchase."

"Provide good peak shape and produces reproducible and reliable results. I would recommend this product."

"Direct comparison of Supelco Discovery C18 (5 µm, 150x4.6mm) and Kinetex C18 (5 µm, 150 x 4.6mm): Analysed the same day on the same instrument (Agilent 1200), using the same sample and mobile phase: Instrument: Agilent 1200 Mobile phase: 20% acetonitrile in 5 mM acetate buffer pH 4.5 (isocratic) Sample: 20 µl of a 100 µg/ml solution (in-house peptide) Flow: 0.5 ml/min Temp: 50°C Retention time Supelco column: 18 minutes Kinetex column: 14 minutes (More retention on the Supelco column, maybe not so surprising) Tailing factor Supelco column: 1.8 Kinetex column: 1.4 (clearly better symmetry on the Kinetex column) Plate count Supelco column: 12.000 plates Kinetex column: 16.000 plates (I am a bit shocked that the Kinetex column gave so much better efficiency. I may have overloaded the Supelco column, giving a lower plate count and more asymmetry) Conclusion: The Kinetex and Supelco columns are probably comparable. The Kinetex column appears to have a better load."

"I recommend to use the pentafluorophenyl columns as they proved to separate phenols and also isomeric phenolic compounds with good efficiency. In our analysis, Oxidation Dyes and direct Dyes are well separated."

"I developed the method and validated it with three different batches of 1.7 µm columns. It was performed on two different HPLC systems and three different analysts. Everything looked fantastic with the lowest USP resolution of the 10 peaks of 3.0 in all runs. The robustness testing showed that I could change pH and acetonitrile concentration in a wide range without any significant loss of resolution. The method is transferred to QC (in another country) without any problems. They buy a large number of columns for the process validation. Only a few works. Peaks are split or very broad. They record the backpressure (very good, 20% less than other competitors)."

"An intermediate mode of separation, between those of Phenyl or C18 columns, of close eluting compounds. Phenomenex offers a pretty good deal on the column, as it is new."

"It's very good and strong column. After more than 2000 injections, the column doesn't give us any problems."

"I have been using several HPLC columns for purification of peptides and peptidomimetics synthesis in my lab and I have never had in my hands a column with such simplicity in its use and endowed with high efficiency! I am using this column for the purification of several kinds of peptides, linear, cyclic and peptidomimetics with great satisfaction!"

"The Kinetex C18 is a column that carries out our analysis in a much faster time than our old column. "

"The lifetime is better than others columns, the time of analyses are reduced and excellent resolution."

"We are very satisfied with the product. The separation efficiency in combination with the release rate is more than adequate. In addition, the column holds many measurements and is very robust. With the service we are very satisfied. Occasionally I get calls with new offers, but not so many that it would be annoying. "

"The Kinetex columns proved to be very easy in the development of the methods and their transfer to other laboratories. They are also very robust because they bear a large number of samples without showing signs of loss of efficiency."

"Very good separation of different peaks, the Peaks are small and tall. Easy handling of the columns, love the new security guard Ultra holder. Using phase C18."

"This is a very different product, very useful to create a new method development & precise results were obtained, just contact with one e-mail. I am very proud to be using this product & special thanks to SelectScience community. "

"I like the innovative idea of using sol-gel processing techniques with Nano structure technology. Additional features such as a porous shell structure minimizes band broadening. This reduction in band broadening results in chromatographic separations with better resolution, higher sensitivity, and improved peak capacities. I think this column is one of the best inventions in the pharmaceutical area."

"We had some problems with the first set-up but when the problem was solved the result was excellent."

"Not a good application in general for protein use. Angstrom sizes are generally too small to allow for sufficient interaction. Sales should focus on wide pore for protein customers. Columns were ordered as a variety based on sales recommendations for getting a variety. "

"The C18 column gave very clean sharp peaks and the chromatography looked great for my tested compound. I would highly recommend this product."

"A very stable column and useful for hydrophobic compounds. "

"A product that gives high performance, but with a short half life."

"This is a very good product because it offers a very good reproducibility in the analysis after having analyzed thousands of samples."

"Allowed for higher sensitivity thereby permitting analysis of diluted samples."

"I have used a number of core shell type columns and am very impressed with the short analysis times achievable on standard LC systems (after some replumbing). In addition, the increased endcapping of the Kinetex XB C18 over the Kinetex C18 allowed me to reduce the formic acid content of my mobile phase when analyzing basic compounds by LC-MS-MS that were previously prone to peak tailing due to secondary interactions with acidic silanols. Sensitivity also improved, due both to narrower peak widths and reduced signal suppression caused by the lower formic acid modifier concentration."

"Breakthrough technology for separations. I had these columns offered to me on a number of occasions to improve my UPLC/MS chromatography, when I finally did it made such a difference to the work we were doing. The sharpness of peaks were unparalleled and we could see low level peaks that were not there with conventional 1.8um columns. Because of the core shell particle the backpressure is much lower also resulting in higher flow rates and even faster more accurate separations. The Kinetex range of columns has now become our go to column for method development and we are branching out to replace old silica columns with the core shell equivalents for our normal HPLC separations."

"The conversion of one method to the method on the Kinetex column was relatively easy. We established better peak shape, better separation and higher resolution with the Kinetex column."

"The column is easy to install to existing fittings of HPLC systems. The performance of the column is excellent when combined with the guard cartridge system, showing no decrease in efficiency or selectivity as yet (>1000 injections). "

"The Kinetex columns are, without a doubt, the single biggest efficiently generator a QC laboratory can introduce. After moving over from standard columns to Kinetex, 90 minute methods were reduced to 26 minutes, solvent costs were substantially reduced and overnight runs could now be completed and reported on the same day and all with no loss in quality. The potential savings to a typical QC lab are staggering!"

"This product is easy to install and easy to use. It is a little expensive."

"I often use Kinetex columns as a first point of call. They're quite robust and durable and provide good separation for most applications. They have a wide pH range, so you don't need to worry about ruining the column with harsh pHs."

"Great peak resolution, sensitivity and a high quality product. Would recommend this product."

"Satisfied with the product, it gives shorter retention times and good peak shapes."

"Good performance, fine resolution and good back pressure."

"This product has a very high resolution power with peaks that are thin and symmetric. A right washing procedure enhances its robustness. "

"My opinion on Kinetex columns (model purchased Kinetex 5u C18 100A) was extremely positive. For determining fat soluble vitamins and other compounds of fat in different biological matrices of animal origin, the results are more than satisfactory. Compared to previous models, both Phenomenex and other trademarks, the Kinetex has outlined peaks with elution times anticipated. This led me to shorten the total time, resulting in a limited consumption of eluent and, ultimately, a tangible economic saving."

"The peak shape is very good, and is very selective for molecules with similar structure and with similar substituents. "

"Great Performance (selectivity, efficiency and resolution), the Core shell technologies are better than others. "

"In LC-MS methods, we successfully used a C18 Kinetex for several applications, including analysis of target peptides from a tryptic digest of a single protein. In the analysis of several mycotoxins it was able to provide good separation in a short time."

"Easy to use and service, with good reproducibility and ability to analyze small samples."

"Versatile, easy to use, lifetime and reproducibility. In summary, one of the best scores for development methods. "

"The column is easy to use with great performance in efficiency, selectivity and resolution. The retention times is reduced by one third compared to a classical column. "

"Provides good tailing and separation, good reproducibility inter-day and inter instruments. The first choice for starting new methods development."

"It is a new wide range application column that gives excellent performance in shorter run cycles."

"The Kinetex C18-XB 100 x 4.6 mm x 2.6 um, line is excellent but more polar phases are missing."

"Product is used for routine antibiotics-screening and non-routine analysis of certain pesticides. It's a robust column, providing sharp peaks with stable retention times, which leads to a short HPLC-method (ca. 20 min). We could change our former 2.6 µm Kinetex column into this 5 µm column without loss of resolution or elongation of the method. The 2.6 µm column got dirty quite fast by small particles that weren't held back by the guard-system and had to be replaced. This is not a problem with the 5 µm column. It is flushed and cleaned easily and thus can be used for a longer time before it has to be replaced. "

"Kinetex phenyl type is tremendously good for the separation of isomers of monophthalates."

"Over the past 25 years I have used various columns and the recent Kinetex columns by Phenomenex are extremely robust and have phenomenal properties to retain polar acidic metabolites. They work well even under fairly acidic conditions (e.g 0.1 % TFA) and the reproducibility of analytes on these is very close to perfect."

"The resolution from this column was wonderful. The separation we received between the desired peaks was very good. The cost was very low, and the sales person even contacted me afterwards to make sure it satisfied our needs!"

"The Kinetex series offers a good broad range of selectivity."

"I have used Kinetex columns with four different stationary phases and they all have been a superior product. Low band broadening effects and good resolution. Also, my contact with Phenomenex has been great when I had questions about stationary phase compatibility."

"I used many Kinetex C18 and HILIC columns in the past several years and these columns work great (good separation, nice peak shape, reliable and very robust)."

"The column was used to shorten a 60 minute method. The results were outstanding as we managed to have an excellent separation in a quarter of the time."

"Great chromatography and excellent after sales help."

"A versatile and robust column which provides repeatable results time and time again."

"Standard column that works well. Used mostly for small molecule prep- using the Kinetex™ 5µm C18 100 Å, AXIA Packed LC Column 250 x 21.2 mm."

"Using the Kinetex 5µm XB-C18 100A met my expectations, which were based on the salespersons' descriptions and recommendations."

"Very easy to use, produces consistent back pressure and retention time."

"The price was perfect, and the sales person was very helpful."

"The Kinetex line of LC columns are easy to use and quite robust. I am pleased with their performance and customer support at Phenomenex."